Several investigators have demonstrated techniques for noninvasive measurement of the oxygen saturation of blood in retinal arteries and veins. These techniques have been based on measuring the optical density of a retinal vessel at multiple wavelengths and on calculating the oxygen saturation on the basis of the known absorption coefficients of hemoglobin and oxyhemoglobin. A technique is presented for determining the optimum wavelengths for retinal oximetry measurements. What is believed to be a novel wavelength combination of 488, 635, and 905 nm is found to provide excellent oxygen sensitivity across a broad range of typical vessel diameters and saturations. The use of this wavelength combination should allow for the most accurate retinal saturation measurements made to date.
© 1999 Optical Society of America
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.4460) Medical optics and biotechnology : Ophthalmic optics and devices
(300.1030) Spectroscopy : Absorption
Matthew H. Smith, "Optimum Wavelength Combinations for Retinal Vessel Oximetry," Appl. Opt. 38, 258-267 (1999)