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Applied Optics

Applied Optics


  • Vol. 38, Iss. 10 — Apr. 1, 1999
  • pp: 2059–2067

Photon cross-correlation spectroscopy to 10-ns resolution

Giuseppe Chirico and Marco Gardella  »View Author Affiliations

Applied Optics, Vol. 38, Issue 10, pp. 2059-2067 (1999)

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We describe a low-cost modification based on the research of Arecchi et al. [Opt. Commun. 3, 284–288 (1971)] on a standard photon correlation spectroscopy setup for measuring small (≃1%) and fast (resolution, ≃10 ns) light fluctuations. Details of the electronics and optics permit the apparatus to be easily reproduced. Careful tests show that spurious correlations of ≤1% can be achieved with standard detectors only when taking precautions with the electronics. Moreover, measurements on latex spheres, ellipsoids, and protein solutions show that fast and very small light fluctuations (≃1-5%) can be studied versus scattering vector and light polarization.

© 1999 Optical Society of America

OCIS Codes
(040.5250) Detectors : Photomultipliers
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(290.1990) Scattering : Diffusion
(290.5850) Scattering : Scattering, particles

Original Manuscript: June 1, 1998
Revised Manuscript: October 5, 1998
Published: April 1, 1999

Giuseppe Chirico and Marco Gardella, "Photon cross-correlation spectroscopy to 10-ns resolution," Appl. Opt. 38, 2059-2067 (1999)

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  1. B. Chu, Laser Light Scattering: Basic Principles and Practice (Academic, London, 1991).
  2. N. L. Thompson, “Fluorescence correlation spectroscopy,” in Techniques, Vol. 1 of Topics in Fluorescence Spectroscopy, J. J. Lakowitz, ed. (Plenum, New York, 1991), pp. 337–378.
  3. B. Berne, R. Pecora, Dynamic Light Scattering (Wiley, New York, 1975).
  4. A. Patkowski, W. Eimer, T. Dorfmüller, “Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering,” Biopolymers 30, 975–983 (1990). [CrossRef]
  5. J. Langowski, W. Kremer, U. Kapp, “Dynamic light scattering for study of solution conformation and dynamics of superhelical DNA,” Methods Enzymol. 211, 430–448 (1992). [PubMed]
  6. G. Chirico, G. Baldini, “Rotational diffusion and internal motions of circular DNA. II. Depolarized photon correlation spectroscopy,” J. Chem. Phys. 104, 6020–6026 (1996). [CrossRef]
  7. P. Schwille, J. Bieschke, F. Oehlenschlager, “Kinetic investigations by fluorescence correlation spectroscopy: the analytical and diagnostic potential of diffusion studies,” Biophys. Chem. 66, 211–228 (1997). [CrossRef] [PubMed]
  8. C. Andries, W. Guedens, J. Clauwert, H. Geerts, “Photon and fluorescence correlation spectroscopy and light scattering of eye-lens proteins at moderate concentrations,” Biophys. J. 43, 345–354 (1983). [CrossRef] [PubMed]
  9. J. R. Abney, B. A. Scalettar, J. C. Owicki, “Mutual diffusion of interacting membrane proteins,” Biophys. J. 56, 315–326 (1990). [CrossRef]
  10. S. Beretta, G. Chirico, D. Arosio, G. Baldini, “Photon correlation spectroscopy of interacting and dissociating hemoglobin,” J. Chem. Phys. 106, 8427–8435 (1997). [CrossRef]
  11. A. Patkowski, W. Eimer, T. Dorfmüller, “A polarized and depolarized dynamic light scattering study of the tRNAPhe conformation in solution,” Biopolymers 30, 93–105 (1990). [CrossRef]
  12. A. Patkowski, S. Jen, B. Chu, “Intensity fluctuation spectroscopy and transfer RNA conformation. Changes of size and shape of tRNA in the melting process,” Biopolymers 17, 2643–2662 (1978). [CrossRef]
  13. A. Patkowski, W. Steffen, H. Nilgens, W. Fischer, R. Pecora, “Depolarized dynamic light scattering from three low molecular weight glass forming liquids: a test of the scattering mechanism,” J. Chem. Phys. 106, 8401–8408 (1997). [CrossRef]
  14. H. Dautet, P. Deschamps, B. Dion, A. D. MacGregor, D. MacSween, R. J. McIntyre, C. Trottier, P. P. Webb, “Photon counting techniques with silicon avalanche photodiodes,” Appl. Opt. 32, 3894–3900 (1993). [PubMed]
  15. R. Foord, R. Jones, C. J. Oliver, E. R. Pike, “The use of photomultiplier tubes for photon counting,” Appl. Opt. 8, 1975–1989 (1969); E. Gulari, B. Chu, “Photon correlation in the nanosecond range and its applications to the evaluation of RCS C31034 photomultiplier tubes,” Rev. Sci. Instrum. 48, 1560–1567 (1977). [CrossRef] [PubMed]
  16. H. C. Burstyn, “Afterpulsing effects in photon correlation experiments,” Rev. Sci. Instrum. 51, 1431–1433 (1980). [CrossRef]
  17. F. T. Arecchi, M. Corti, V. Degiorgio, S. Donati, “Measurements of the light intensity correlations in the subnanosecond region by photomultipliers,” Opt. Commun. 3, 284–288 (1971). [CrossRef]
  18. C. Bendjaballah, “Autocorrelation function of scattered light for a binary fluid near the critical mixing point,” Opt. Commun. 9, 279–281 (1973). [CrossRef]
  19. D. A. Weitz, D. J. Pine, P. N. Pusey, R. J. A. Tough, “Nondiffusive Brownian motion studied by diffusing-wave spectroscopy,” Phys. Rev. Lett. 63, 1747–1750 (1989). [CrossRef] [PubMed]
  20. B. Hinz, G. Simonsohn, M. Hendrix, G. Wu, A. Leipertz, “The superposition of Rayleigh and Brillouin radiation in photon correlation spectroscopy of liquids,” J. Mod. Opt. 34, 1093–1106 (1987). [CrossRef]
  21. H. C. Burstyn, J. V. Sengers, “Time dependence of critical concentration fluctuations in a binary liquid,” Phys. Rev. A 27, 1071–1085 (1983). [CrossRef]
  22. G. D. J. Phillies, “Suppression of multiple scattering effects in quasi-elastic light scattering by homodyne cross-correlation techniques,” J. Chem. Phys. 74, 260–262 (1981); “Experimental demonstration of multiple-scattering suppression in quasi-elastic-light-scattering spectroscopy by homodyne coincidence techniques,” Phys. Rev. A 24, 1939–1943 (1981). [CrossRef]
  23. J. K. G. Dhont, C. G. de Kruif, “Scattered light intensity cross correlation. I. Theory,” J. Chem. Phys. 79, 1658–1663 (1983); H. J. Mos, C. Pathmamanoharan, J. K. G. Dhont, C. G. de Kruif, “Scattered light intensity cross correlation. II. Experimental,” J. Chem. Phys. 84, 45–49 (1986). [CrossRef]
  24. G. Nägele, “On the dynamics and structure of charge-stabilized suspensions,” Phys. Rep. 272, 215–372 (1996). [CrossRef]
  25. P. N. Segre, W. Van Menegen, P. N. Pusey, W. Peters, “Two-color dynamic light scattering,” J. Mod. Opt. 42, 1929–1952 (1995). [CrossRef]
  26. We are referring to the Small Outline Single Photon Detector setup produced by ALV-Laser mbH, Langen, Germany.
  27. R. Peters, “Introduction to the multiple tau correlator Technique,” (ALV-Laser mbH, Langen, Germany).
  28. K. Schätzel, “Noise on photon correlation data: I,” Quantum Opt. 2, 287–305 (1990). [CrossRef]
  29. R. Piazza, J. Stavans, T. T. Bellini, V. De Giorgio, “Light scattering study of crystalline latex particles,” Opt. Commun. 73, 263–267 (1989). [CrossRef]
  30. G. Phillies, G. Benedek, N. Mazer, “Diffusion in protein solutions at high concentrations: a study by quasi-elastic light scattering spectroscopy,” J. Chem. Phys. 65, 1883–1892 (1976). [CrossRef]
  31. S. B. Dubin, N. A. Clark, G. B. Benedek, “Measurement of the rotational diffusion coefficient of lysozyme by depolarized light scattering: configuration of lysozyme in solution,” J. Chem. Phys. 54, 5158–5164 (1971). [CrossRef]
  32. M. Muschol, F. Rosenberger, “Interactions in undersaturated and supersaturated lysozyme solutions: static and dynamic light scattering results,” J. Chem. Phys. 103, 10424–10432 (1995). [CrossRef]
  33. D. E. Kuehner, C. Heyer, C. Rämsch, U. M. Fornefeld, H. W. Blanch, J. M. Prausnitz, “Interactions of lysozyme in concentrated electrolyte solutions from dynamic light-scattering measurements,” Biophys. J. 73, 3211–3224 (1997). [CrossRef] [PubMed]

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