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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 38, Iss. 12 — Apr. 20, 1999
  • pp: 2524–2535

Theoretical treatment of fluorescence detection by a dual-fiber-optic sensor with consideration of sampling variability and package effects associated with particles

Eurico J. D’Sa and Steven E. Lohrenz  »View Author Affiliations


Applied Optics, Vol. 38, Issue 12, pp. 2524-2535 (1999)
http://dx.doi.org/10.1364/AO.38.002524


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Abstract

The characteristics of a dual-fiber-optic sensor for measurements of chlorophyll fluorescence in aquatic environments were evaluated with a theoretical model. Consideration was given to sampling variability and package effects associated with particles (e.g., phytoplankton cells). A numerical simulation was developed to approximate the optical geometry of the dual fiber-optic sensor that permitted a visual representation of the fluorescence distribution within the sensor sampling volume. A Monte Carlo simulation was used to evaluate sampling variability associated with the number and distribution of particles within the sampling volume. Relatively high coefficients of variation were associated with low particle concentrations, although with sufficient signal averaging the coefficient of variation was reduced to less than 20%. The influence of package effects and intracellular absorption of fluorescence was evaluated with a simplified form of the model that treated fluorescence as a linear function of particle density and assumed uniform particle composition, constant fluorescence cross-sectional yield, and sufficient averaging of the fluorescence signal. The model predicted decreasing fluorescence per unit of chlorophyll with increasing values of the product of particle diameter and intraparticle chlorophyll concentration. Experimental trends in size dependence of chlorophyll–fluorescence relationships were compared with predictions of the model.

© 1999 Optical Society of America

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(060.2310) Fiber optics and optical communications : Fiber optics
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(120.4570) Instrumentation, measurement, and metrology : Optical design of instruments
(170.1420) Medical optics and biotechnology : Biology
(260.2510) Physical optics : Fluorescence

History
Original Manuscript: September 14, 1998
Revised Manuscript: January 6, 1999
Published: April 20, 1999

Citation
Eurico J. D’Sa and Steven E. Lohrenz, "Theoretical treatment of fluorescence detection by a dual-fiber-optic sensor with consideration of sampling variability and package effects associated with particles," Appl. Opt. 38, 2524-2535 (1999)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-38-12-2524


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References

  1. V. Strass, “On the calibration of large-scale fluorometric chlorophyll measurements from towed undulating vehicles,” Deep-Sea Res. 37, 525–540 (1990). [CrossRef]
  2. C. Wirick, “Exchange of phytoplankton across the continental shelf-slope boundary of the Middle Atlantic Bight during spring 1988,” Deep-Sea Res. 41, 391–410 (1994). [CrossRef]
  3. D. A. Kiefer, “Fluorescence properties of natural phytoplankton populations,” Mar. Biol. 22, 263–269 (1973). [CrossRef]
  4. A. E. Alpine, J. E. Cloern, “Differences in in vivo fluorescence yield between three phytoplankton size classes,” J. Plankton Res. 7, 381–390 (1985). [CrossRef]
  5. D. J. Collins, D. A. Kiefer, J. B. SooHoo, I. S. McDermid, “The role of reabsorption in the spectral distribution of phytoplankton fluorescence emission,” Deep-Sea Res. 32, 983–1003 (1985). [CrossRef]
  6. P. Falkowski, D. A. Kiefer, “Chlorophyll a fluorescence in phytoplankton: relationship to photosynthesis and biomass,” J. Plankton Res. 7, 715–731 (1985). [CrossRef]
  7. A. Morel, A. Bricaud, “Inherent optical properties of algal cells including picoplankton: theoretical and experimental results,” Can. Bull. Fish. Aquat. Sci. 214, 521–559 (1986).
  8. B. G. Mitchell, D. A. Kiefer, “Chlorophyll a specific absorption and fluorescence excitation spectra for light-limited phytoplankton,” Deep-Sea Res. 35, 639–663 (1988). [CrossRef]
  9. G. Papageorgio, “Chlorophyll fluorescence: an intrinsic probe of photosynthesis,” in Bioenergetics of Photosynthesis, R. Govindjee, ed. (Academic, New York, 1975), pp. 319–371.
  10. K. K. Karukstis, “Chlorophyll fluorescence as a physiological probe of the photosynthetic apparatus,” in Chlorophylls, H. Scheer, ed. (CRC Press, Boca Raton, Fla., 1991), pp. 769–795.
  11. Z. Kolber, P. G. Falkowski, “Use of active fluorescence to estimate phytoplankton photosynthesis in situ,” Limnol. Oceanogr. 38, 1646–1665 (1993). [CrossRef]
  12. T. J. Cowles, R. A. Desiderio, “Resolution of biological microstructure through in situ fluorescence emission spectra,” Oceanography 6, 105–111 (1993). [CrossRef]
  13. R. A. Desiderio, T. J. Cowles, J. N. Moum, “Microstructure profiles of laser-induced chlorophyll fluorescence spectra: evaluation of backscatter and forward-scatter fiber-optic sensors,” J. Atmos. Ocean. Technol. 10, 209–224 (1993). [CrossRef]
  14. E. J. D’Sa, S. E. Lohrenz, V. L. Asper, R. A. Walters, “Time series measurements of chlorophyll fluorescence in the oceanic bottom boundary layer with a multisensor fiber-optic fluorometer,” J. Atmos. Ocean. Technol. 14, 889–896 (1997). [CrossRef]
  15. E. J. D’Sa, S. E. Lohrenz, V. L. Asper, R. A. Walters, M. J. Morris, C. Rathbun, “A multi-sensor in situ fiber-optic fluorometer,” in Ocean Optics XII, J. S. Jaffe, ed., Proc. SPIE2258, 793–796 (1994).
  16. P. Plaza, N. Q. Dao, M. Jouan, H. Fevrier, H. Saisse, “Simulation et optimisation des capteurs a fibres optiques adjacentes,” Appl. Opt. 25, 3448–3454 (1986). [CrossRef]
  17. H. R. Gordon, K. J. Voss, K. A. Kilpatrick, “Angular distribution of fluorescence from phytoplankton,” Limnol. Oceanogr. 38, 1582–1586 (1993). [CrossRef]
  18. A. Morel, A. Bricaud, “Theoretical results concerning light absorption in a discrete medium, and application to specific absorption of phytoplankton,” Deep-Sea Res. 28A, 1375–1393 (1981). [CrossRef]
  19. J. T. O. Kirk, Light and Photosynthesis in Aquatic Ecosystems, 2nd ed. (Cambridge U. Press, New York, 1994). [CrossRef]
  20. R. J. Geider, B. A. Osborne, “Light absorption by a marine diatom: experimental observations and theoretical calculations of the package effect in a small Thalassiosira species,” Mar. Biol. 96, 299–308 (1987). [CrossRef]
  21. H. R. Gordon, “Diffuse reflectance of the ocean: the theory of its augmentation by chlorophyll a fluorescence at 685 nm,” Appl. Opt. 18, 1161–1166 (1979). [CrossRef] [PubMed]
  22. N. Hoepffner, S. Sathyendranath, “Determination of the major groups of phytoplankton pigments from the absorption spectra of total particulate matter,” J. Geophys. Res. 98, 22,789–22,803 (1993). [CrossRef]
  23. B. G. Mitchell, D. A. Kiefer, “Variability in pigment specific particulate fluorescence and absorption spectra in the northeastern Pacific Ocean,” Deep-Sea Res. 35, 665–689 (1988). [CrossRef]
  24. S. Sathyendranath, L. Lazzara, L. Prieur, “Variations in the spectral values of specific absorption of phytoplankton,” Limnol. Oceanogr. 32, 403–415 (1987). [CrossRef]
  25. A. Bricaud, A.-L. Bédhomme, A. Morel, “Optical properties of diverse phytoplanktonic species: experimental results and theoretical interpretation,” J. Plank. Res. 10, 851–873 (1988). [CrossRef]
  26. Y. Ahn, A. Bricaud, A. Morel, “Light backscattering efficiency and related properties of some phytoplankters,” Deep-Sea Res. 39, 1835–1855 (1992). [CrossRef]
  27. G. Johnsen, E. Sakshaug, “Bio-optical characteristics and photoadaptive responses in the toxic and bloom-forming dinoflagellates Gyrodinium aureolum, Gyrodinium galatheanum, and two strains of Prorocentrum minimum,” J. Phycol. 29, 627–642 (1993). [CrossRef]
  28. R. R. L. Guillard, “Culture of phytoplankton for feeding marine invertebrates,” in Culture of Marine Invertebrate Animals, W. L. Smith, M. H. Chanley, eds. (Plenum, New York, 1975), pp. 29–60. [CrossRef]
  29. P. G. Falkowski, J. A. Raven, Aquatic Photosynthesis (Blackwell Science, Malden, Mass., 1997).
  30. E. J. D’Sa, “Pigment dynamics in a coastal bottom boundary layer and its relation to the physical regime: measurements using an in situ fiber-optic fluorometer,” Ph.D. dissertation (University of Southern Mississippi, Hattiesburg, Miss., 1996), p. 185.

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