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Applied Optics

Applied Optics


  • Vol. 38, Iss. 12 — Apr. 20, 1999
  • pp: 2524–2535

Theoretical treatment of fluorescence detection by a dual-fiber-optic sensor with consideration of sampling variability and package effects associated with particles

Eurico J. D’Sa and Steven E. Lohrenz  »View Author Affiliations

Applied Optics, Vol. 38, Issue 12, pp. 2524-2535 (1999)

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The characteristics of a dual-fiber-optic sensor for measurements of chlorophyll fluorescence in aquatic environments were evaluated with a theoretical model. Consideration was given to sampling variability and package effects associated with particles (e.g., phytoplankton cells). A numerical simulation was developed to approximate the optical geometry of the dual fiber-optic sensor that permitted a visual representation of the fluorescence distribution within the sensor sampling volume. A Monte Carlo simulation was used to evaluate sampling variability associated with the number and distribution of particles within the sampling volume. Relatively high coefficients of variation were associated with low particle concentrations, although with sufficient signal averaging the coefficient of variation was reduced to less than 20%. The influence of package effects and intracellular absorption of fluorescence was evaluated with a simplified form of the model that treated fluorescence as a linear function of particle density and assumed uniform particle composition, constant fluorescence cross-sectional yield, and sufficient averaging of the fluorescence signal. The model predicted decreasing fluorescence per unit of chlorophyll with increasing values of the product of particle diameter and intraparticle chlorophyll concentration. Experimental trends in size dependence of chlorophyll–fluorescence relationships were compared with predictions of the model.

© 1999 Optical Society of America

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(060.2310) Fiber optics and optical communications : Fiber optics
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(120.4570) Instrumentation, measurement, and metrology : Optical design of instruments
(170.1420) Medical optics and biotechnology : Biology
(260.2510) Physical optics : Fluorescence

Original Manuscript: September 14, 1998
Revised Manuscript: January 6, 1999
Published: April 20, 1999

Eurico J. D’Sa and Steven E. Lohrenz, "Theoretical treatment of fluorescence detection by a dual-fiber-optic sensor with consideration of sampling variability and package effects associated with particles," Appl. Opt. 38, 2524-2535 (1999)

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