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Applied Optics

Applied Optics


  • Vol. 38, Iss. 13 — May. 1, 1999
  • pp: 2916–2926

Multicomponent blood analysis by near-infrared Raman spectroscopy

Andrew J. Berger, Tae-Woong Koo, Irving Itzkan, Gary Horowitz, and Michael S. Feld  »View Author Affiliations

Applied Optics, Vol. 38, Issue 13, pp. 2916-2926 (1999)

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We demonstrate the use of Raman spectroscopy to measure the concentration of many important constituents (analytes) in serum and whole blood samples at physiological concentration in vitro across a multipatient data set. A near-infrared (830-nm) diode laser generates Raman spectra that contain superpositions of Raman signals from different analytes. Calibrations for glucose, cholesterol, urea, and other analytes are developed by use of partial least-squares cross validation. We predict six analytes in serum with significant accuracy in a 66-patient data set, using 60-s spectra. The calibrations are shown to be fairly robust against system drift over the span of seven weeks. In whole blood, a preliminary analysis yields accurate predictions of some of the same analytes and also hematocrit. The results hold promise for potential medical applications.

© 1999 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.5660) Medical optics and biotechnology : Raman spectroscopy

Original Manuscript: July 29, 1998
Revised Manuscript: February 8, 1999
Published: May 1, 1999

Andrew J. Berger, Tae-Woong Koo, Irving Itzkan, Gary Horowitz, and Michael S. Feld, "Multicomponent blood analysis by near-infrared Raman spectroscopy," Appl. Opt. 38, 2916-2926 (1999)

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