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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 38, Iss. 16 — Jun. 1, 1999
  • pp: 3644–3650

Estimation of Transmittance Spectra from Multiband Micrographs of Fungi and its Application to Segmentation of Conidia and Hyphae

Masaru Takeya, Norimichi Tsumura, Hideaki Haneishi, and Yoichi Miyake  »View Author Affiliations


Applied Optics, Vol. 38, Issue 16, pp. 3644-3650 (1999)
http://dx.doi.org/10.1364/AO.38.003644


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Abstract

Transmittance spectra of fungi were estimated from seven-band optical micrographs. An optical microscope adjusted with a single-chip CCD camera with seven band filters was used for image acquisition. The Wiener method was applied to estimate the transmittance spectra of five species that belong to one genus of fungi. The Wiener estimation operator was calculated from transmittance spectra of 16 color transparencies and the corresponding camera responses. The estimated transmittance spectra were used for segmentation of conidia and hyphae in fungal optical micrographs; then the competitive learning in an artificial neural network was applied to the segmentation.

© 1999 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0180) Medical optics and biotechnology : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging

Citation
Masaru Takeya, Norimichi Tsumura, Hideaki Haneishi, and Yoichi Miyake, "Estimation of Transmittance Spectra from Multiband Micrographs of Fungi and its Application to Segmentation of Conidia and Hyphae," Appl. Opt. 38, 3644-3650 (1999)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-38-16-3644


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