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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 38, Iss. 16 — Jun. 1, 1999
  • pp: 3662–3669

Fluorescence measurement in thick tissue layers by linear or nonlinear long-wavelength excitation

Peter Lenz  »View Author Affiliations


Applied Optics, Vol. 38, Issue 16, pp. 3662-3669 (1999)
http://dx.doi.org/10.1364/AO.38.003662


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Abstract

Samples of different animal tissues containing, at variable depth, a thin fluorescent sheet are irradiated with continuous violet or red light or with nonlinearly absorbed pulsed infrared light. The fluorescence intensity measured at the tissue surface as a function of the location of the fluorescent sheet exhibits, after a transition zone close to the tissue surface, an exponential decrease, the slope of which depends on the optical penetration depths of the exciting and the fluorescent light. From these results the total fluorescence output is determined for specific fluorophor distributions. It is seen that considerably deeper tissue layers are explored by use of excitation with red instead of violet light. Nonlinear excitation by infrared light can provide a further improvement, especially in liver tissue.

© 1999 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6930) Medical optics and biotechnology : Tissue
(170.7050) Medical optics and biotechnology : Turbid media
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
(190.4180) Nonlinear optics : Multiphoton processes
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials

History
Original Manuscript: November 3, 1998
Revised Manuscript: February 5, 1999
Published: June 1, 1999

Citation
Peter Lenz, "Fluorescence measurement in thick tissue layers by linear or nonlinear long-wavelength excitation," Appl. Opt. 38, 3662-3669 (1999)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-38-16-3662

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