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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 38, Iss. 19 — Jul. 1, 1999
  • pp: 4217–4227

Integrating the digitized backscattered image to measure absorption and reduced-scattering coefficients in vivo

Laure Gobin, Loïc Blanchot, and Hervé Saint-Jalmes  »View Author Affiliations


Applied Optics, Vol. 38, Issue 19, pp. 4217-4227 (1999)
http://dx.doi.org/10.1364/AO.38.004217


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Abstract

Measurement of absorption and reduced-scattering optical coefficients μ a and μ s ′ is possible when a steady-state backscattered image is used on a sample surface. A new method for processing the backscattered image, acquired with a CCD, has been developed. The image is integrated to decrease sensitivity to noise. The resulting curve is defined as the integral reflectance. The curve is then fitted with a relaxation model to evaluate μ a and μ s ′. We have validated the method with calibrated scattering and absorption phantoms. The integral reflectance method is then applied to measurements of the μ a and μ s ′ coefficients of human skin in vivo.

© 1999 Optical Society of America

OCIS Codes
(040.1520) Detectors : CCD, charge-coupled device
(170.6930) Medical optics and biotechnology : Tissue
(290.0290) Scattering : Scattering
(300.1030) Spectroscopy : Absorption

History
Original Manuscript: September 8, 1998
Revised Manuscript: February 9, 1999
Published: July 1, 1999

Citation
Laure Gobin, Loïc Blanchot, and Hervé Saint-Jalmes, "Integrating the digitized backscattered image to measure absorption and reduced-scattering coefficients in vivo," Appl. Opt. 38, 4217-4227 (1999)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-38-19-4217

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