Measurement of absorption and reduced-scattering optical coefficients μ a and μ s ′ is possible when a steady-state backscattered image is used on a sample surface. A new method for processing the backscattered image, acquired with a CCD, has been developed. The image is integrated to decrease sensitivity to noise. The resulting curve is defined as the integral reflectance. The curve is then fitted with a relaxation model to evaluate μ a and μ s ′. We have validated the method with calibrated scattering and absorption phantoms. The integral reflectance method is then applied to measurements of the μ a and μ s ′ coefficients of human skin in vivo.
© 1999 Optical Society of America
Original Manuscript: September 8, 1998
Revised Manuscript: February 9, 1999
Published: July 1, 1999
Laure Gobin, Loïc Blanchot, and Hervé Saint-Jalmes, "Integrating the digitized backscattered image to measure absorption and reduced-scattering coefficients in vivo," Appl. Opt. 38, 4217-4227 (1999)