A confocal microscope that uses separate, noncollinear objective lenses for illumination and collection makes it possible to work 20 mm from a sample with a field of view of 2.25 mm across and still achieve lateral and axial resolutions below 5 μm. The design is expected to have application to <i>in vivo</i> confocal microscopy, allowing a field of view and section thickness similar to those used by pathologists in examining excised tissue.
© 1999 Optical Society of America
(110.0180) Imaging systems : Microscopy
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.0180) Medical optics and biotechnology : Microscopy
(180.1790) Microscopy : Confocal microscopy
Robert H. Webb and Fran Rogomentich, "Confocal Microscope with Large Field and Working Distance," Appl. Opt. 38, 4870-4875 (1999)