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Applied Optics

Applied Optics


  • Vol. 38, Iss. 22 — Aug. 1, 1999
  • pp: 4870–4875

Confocal microscope with large field and working distance

Robert H. Webb and Fran Rogomentich  »View Author Affiliations

Applied Optics, Vol. 38, Issue 22, pp. 4870-4875 (1999)

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A confocal microscope that uses separate, noncollinear objective lenses for illumination and collection makes it possible to work 20 mm from a sample with a field of view of 2.25 mm across and still achieve lateral and axial resolutions below 5 µm. The design is expected to have application to in vivo confocal microscopy, allowing a field of view and section thickness similar to those used by pathologists in examining excised tissue.

© 1999 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.0180) Medical optics and biotechnology : Microscopy
(180.1790) Microscopy : Confocal microscopy

Original Manuscript: January 4, 1999
Revised Manuscript: May 5, 1999
Published: August 1, 1999

Robert H. Webb and Fran Rogomentich, "Confocal microscope with large field and working distance," Appl. Opt. 38, 4870-4875 (1999)

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