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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 38, Iss. 3 — Jan. 20, 1999
  • pp: 486–494

Unmixing coral fluorescence emission spectra and predicting new spectra under different excitation conditions

Eran Fux and Charles Mazel  »View Author Affiliations


Applied Optics, Vol. 38, Issue 3, pp. 486-494 (1999)
http://dx.doi.org/10.1364/AO.38.000486


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Abstract

An algorithm was developed that uses prototype spectra and least-squares minimization to unmix the relative contributions of individual pigments to the composite fluorescence emission spectrum of reef corals. Field measurements indicated that it was necessary to include allowance for spectral shift of the wavelength peak of the prototype emission spectra. The unmixed spectra are used to predict the shape and amplitude of composite spectra that would be expected under different excitation conditions. We found that, for cases in which the pigments occur singly or with significant spectral separation, it is necessary to consider the properties of the excitation light sources, only, to make accurate predictions. In cases with spectral overlap the contribution of interpigment coupling cannot be neglected.

© 1999 Optical Society of America

OCIS Codes
(010.0010) Atmospheric and oceanic optics : Atmospheric and oceanic optics
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

History
Original Manuscript: May 18, 1998
Revised Manuscript: September 21, 1998
Published: January 20, 1999

Citation
Eran Fux and Charles Mazel, "Unmixing coral fluorescence emission spectra and predicting new spectra under different excitation conditions," Appl. Opt. 38, 486-494 (1999)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-38-3-486


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References

  1. C. J. Lorenzen, “A method for the continuous measurement of in vivo chlorophyll concentration,” Deep-Sea Res. 13, 223–227 (1966).
  2. T. J. Cowles, R. A. Desiderio, S. Neuer, “In situ characterization of phytoplankton from vertical profiles of fluorescence emission spectra,” Mar. Biol. 115, 217–222 (1993). [CrossRef]
  3. J. Hardy, F. Hoge, J. Yungel, R. Dodge, “Remote detection of coral bleaching using pulsed-laser fluorescence spectroscopy,” Mar. Ecol. Prog. Ser. 88, 247–255 (1992). [CrossRef]
  4. J. A. Topinka, K. Korjeff Bellows, C. S. Yentch, “Characterization of marine macroalgae by fluorescence signatures,” Int. J. Remote Sensing 11, 2329–2335 (1990). [CrossRef]
  5. S. Kawaguti, “On the physiology of reef corals VI: study on the pigments,” Palao Trop. Biol. Stn. Stud. 2, 617–674 (1944).
  6. R. Catala, “Fluorescence effects from corals irradiated with ultra-violet rays,” Nature (London) 183, 949 (1959). [CrossRef]
  7. K. Shibata, “Pigments and a UV-absorbing substance in corals and a blue-green alga living in the Great Barrier Reef,” Plant Cell Physiol. 10, 325–335 (1969).
  8. P. L. Jokiel, R. H. York, “Solar ultraviolet photobiology of the reef coral Pocillopora damicornis and symbiotic zooxanthellae,” Bull. Mar. Sci. 32, 301–315 (1982).
  9. D. Schlichter, H. W. Fricke, W. Weber, “Light harvesting by wavelength transformation in a symbiotic coral in the Red Sea twilight zone,” Mar. Biol. 91, 403–407 (1986). [CrossRef]
  10. A. Logan, K. Halcrow, T. Tomascik, “UV excitation-fluorescence in polyp tissue of certain scleractinian corals from Barbados and Bermuda,” Bull. Mar. Sci. 46, 807–813 (1990).
  11. L. Delvoye, “Endolithic algae in living stony corals: algal concentrations under influence of depth-dependent light conditions and coral tissue fluorescence in Agaricia agaricites (L.) and Meandrina meandrites (L.) (Scleractinia, Anthozoa),” Stud. Nat. Hist. Caribbean Region 71, 24–41 (1992).
  12. D. F. Gleason, “Differential effects of ultraviolet radiation on green and brown morphs of the Caribbean coral Porites astreoides,” Limnol. Oceanogr. 38, 1452–1463 (1993). [CrossRef]
  13. C. Mazel, “Spectral measurements of fluorescence emission in Caribbean cnidarians,” Mar. Ecol. Prog. Ser. 120, 185–191 (1995). [CrossRef]
  14. C. Mazel, “Coral fluorescence characteristics: excitation/emission spectra, fluorescence efficiencies, and contribution to apparent reflectance,” in Ocean Optics XIII, S. G. Ackleson, R. Frouin, eds., Proc. SPIE2963, 240–245 (1997). [CrossRef]
  15. C. Mazel, “Diver-operated instrument for in-situ measurement of spectral fluorescence and reflectance of benthic marine organisms and substrates,” Opt. Eng. 36, 2612–2617 (1997). [CrossRef]
  16. M. R. Myers, J. T. Hardy, C. H. Mazel, P. Dustan, “Optical spectra and pigmentation of Caribbean reef corals and macroalgae, Coral Reefs (to be published).
  17. M. P. Strand, B. W. Coles, A. J. Nevis, R. Regan, “Laser line-scan fluorescence and multispectral imaging of coral reef environment,” in Ocean Optics XIII, S. G. Ackleson, R. Frouin, eds., Proc. SPIE2963, 790–795 (1997).
  18. A. Savitzky, M. J. E. Golay, “Smoothing and differentiation of data by simplified least squares procedure,” Anal. Chem. 36, 1627–1639 (1964). [CrossRef]
  19. G. Strang, Introduction to Applied Mathematics (Wellesley-Cambridge Press, Wellesley, Mass., 1986).
  20. J. J. Settele, N. A. Drake, “Linear mixing and the estimation of ground cover proportions,” Int. J. Remote Sensing 14, 1159–1177 (1993). [CrossRef]
  21. C. D. Mobley, Light and Water (Academic, San Diego, Calif., 1994).
  22. J. C. Goedheer, “Fluorescence in relation to photosynthesis,” Ann. Rev. Plant Physiol. 23, 87–112 (1972). [CrossRef]

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