We demonstrate a three-dimensional scanning probe microscope in which the extremely soft spring of an optical tweezers trap is used. Feedback control of the instrument based on backscattered light levels allows three-dimensional imaging of microscopic samples in an aqueous environment. Preliminary results with a 2-μm-diameter spherical probe indicate that features of approximately 200 nm can be resolved, with a sensitivity of 5 nm in the height measurement. The theoretical resolution is limited by the probe dimensions.
© 1999 Optical Society of America
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.0110) Medical optics and biotechnology : Imaging systems
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(180.5810) Microscopy : Scanning microscopy
M. E. J. Friese, A. G. Truscott, H. Rubinsztein-Dunlop, and N. R. Heckenberg, "Three-Dimensional Imaging with Optical Tweezers," Appl. Opt. 38, 6597-6603 (1999)