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Applied Optics

Applied Optics


  • Vol. 38, Iss. 31 — Nov. 1, 1999
  • pp: 6597–6603

Three-dimensional imaging with optical tweezers

M. E. J. Friese, A. G. Truscott, H. Rubinsztein-Dunlop, and N. R. Heckenberg  »View Author Affiliations

Applied Optics, Vol. 38, Issue 31, pp. 6597-6603 (1999)

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We demonstrate a three-dimensional scanning probe microscope in which the extremely soft spring of an optical tweezers trap is used. Feedback control of the instrument based on backscattered light levels allows three-dimensional imaging of microscopic samples in an aqueous environment. Preliminary results with a 2-µm-diameter spherical probe indicate that features of approximately 200 nm can be resolved, with a sensitivity of 5 nm in the height measurement. The theoretical resolution is limited by the probe dimensions.

© 1999 Optical Society of America

OCIS Codes
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.0110) Medical optics and biotechnology : Imaging systems
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(180.5810) Microscopy : Scanning microscopy

Original Manuscript: January 15, 1999
Revised Manuscript: July 20, 1999
Published: November 1, 1999

M. E. J. Friese, A. G. Truscott, H. Rubinsztein-Dunlop, and N. R. Heckenberg, "Three-dimensional imaging with optical tweezers," Appl. Opt. 38, 6597-6603 (1999)

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