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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 38, Iss. 31 — Nov. 1, 1999
  • pp: 6628–6637

Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo

George Zonios, Lev T. Perelman, Vadim Backman, Ramasamy Manoharan, Maryann Fitzmaurice, Jacques Van Dam, and Michael S. Feld  »View Author Affiliations


Applied Optics, Vol. 38, Issue 31, pp. 6628-6637 (1999)
http://dx.doi.org/10.1364/AO.38.006628


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Abstract

Diffuse reflectance spectra were collected from adenomatous colon polyps (cancer precursors) and normal colonic mucosa of patients undergoing colonoscopy. We analyzed the data by using an analytical light diffusion model, which was tested and validated on a physical tissue model composed of polystyrene beads and hemoglobin. Four parameters were obtained: hemoglobin concentration, hemoglobin oxygen saturation, effective scatterer density, and effective scatterer size. Normal and adenomatous tissue sites exhibited differences in hemoglobin concentration and, on average, in effective scatterer size, which were in general agreement with other studies that employ standard methods. These results suggest that diffuse reflectance can be used to obtain tissue information about tissue structure and composition in vivo.

© 1999 Optical Society of America

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.7050) Medical optics and biotechnology : Turbid media
(290.4020) Scattering : Mie theory

History
Original Manuscript: March 15, 1999
Revised Manuscript: July 12, 1999
Published: November 1, 1999

Citation
George Zonios, Lev T. Perelman, Vadim Backman, Ramasamy Manoharan, Maryann Fitzmaurice, Jacques Van Dam, and Michael S. Feld, "Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo," Appl. Opt. 38, 6628-6637 (1999)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-38-31-6628


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