OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 38, Iss. 31 — Nov. 1, 1999
  • pp: 6648–6652

Observation of the dynamics of live cardiomyocytes through a free-running scanning near-field optical microscopy setup

Ruggero Micheletto, Morgan Denyer, Martin Scholl, Ken Nakajima, Andreas Offenhauser, Masahiko Hara, and Wolfgang Knoll  »View Author Affiliations


Applied Optics, Vol. 38, Issue 31, pp. 6648-6652 (1999)
http://dx.doi.org/10.1364/AO.38.006648


View Full Text Article

Enhanced HTML    Acrobat PDF (635 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We report the observation of live-cell dynamics by noncontact scanning near-field optical microscopy (SNOM) modified to work with living biological samples that are fully immersed in liquid. We did not use the SNOM setup in strictly near-field conditions (we used 1-µm constant-height mode); however, we could examine the dynamics of rhythmically beating cardiac myocytes in culture with extremely high vertical sensitivity below the nanometric range. We could halt scans at any point to record localized contraction profiles of the cell membrane. We show that the contractions of the organisms changed shape dramatically within adjacent areas. We believe that the spatial dependency of the contractions arises because of the measurement system’s ability to resolve the behavior of individual submembrane actin bundles. Our results, combining imaging and real-time recording in localized areas, reveal a new, to our knowledge, noninvasive method for using SNOM setups for studying the dynamics of live biological samples.

© 1999 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.5810) Microscopy : Scanning microscopy

History
Original Manuscript: February 25, 1999
Revised Manuscript: June 14, 1999
Published: November 1, 1999

Citation
Ruggero Micheletto, Morgan Denyer, Martin Scholl, Ken Nakajima, Andreas Offenhauser, Masahiko Hara, and Wolfgang Knoll, "Observation of the dynamics of live cardiomyocytes through a free-running scanning near-field optical microscopy setup," Appl. Opt. 38, 6648-6652 (1999)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-38-31-6648


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. E. Betzig, J. K. Trautman, T. D. Harris, J. S. Weiner, R. L. Kostelak, “Breaking the diffraction barrier: optical microscopy on a nanometric scale,” Science 251, 1468–1470 (1991). [CrossRef] [PubMed]
  2. M. Ohtsu, “Photon STM: from imaging to fabrication,” Optoelectron. Devices Technol. 10, 147–166 (1995).
  3. A. Shrier, J. R. Clay, “Pacemaker currents in chick embryonic heart cells change with development,” Nature 283, 670–671 (1980). [CrossRef] [PubMed]
  4. S. Mononobe, M. Naya, T. Saiki, M. Ohtsu, “Reproducible fabrication of a fiber probe with a nanometric protrusion for near-field optics,” Appl. Opt. 36, 1496–1500 (1997). [CrossRef] [PubMed]
  5. T. Saiki, S. Mononobe, M. Ohtsu, N. Saito, K. Kusano, “Tailoring a high-transmission fiber probe for photon scanning tunneling microscope,” Appl. Phys. Lett. 68, 2612–2614 (1996). [CrossRef]
  6. J. H. Rose, M. R. Kaufmann, S. A. Wickline, C. S. Hall, J. G. Miller, “A proposed microscopic elastic wave theory for ultrasonic backscatter from myocardial tissue,” J. Acoust. Soc. Am. 97, 656–668 (1995). [CrossRef] [PubMed]
  7. J. Meunier, M. Bertrand, “Echographic image mean gray level changes with tissue dynamics: a system-based model study,” IEEE Trans. Biomed. Eng. 42, 403–410 (1995). [CrossRef] [PubMed]
  8. R. U. Maheswari, S. Mononobe, H. Tatsumi, Y. Katayama, M. Ohtsu, “Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control,” Opt. Rev. 3, 463–467 (1996). [CrossRef]
  9. M. F. Arnsdorf, R. Lal, “Recent progress with atomic force microscopy in biology: molecular resolution imaging of cell membranes, constituent biomolecules, and microcrystals,” in Imaging Technologies and Applications, R. J. Heaston, ed., Proc. SPIE1778, 112–16 (1992). [CrossRef]
  10. R. U. Maheswari, H. Kadono, M. Ohtsu, “Power spectral analysis for evaluating optical near-field images of 20 nm gold particles,” Opt. Commun. 131, 133–142 (1996). [CrossRef]
  11. B. Bailey, D. L. Farkas, D. L. Taylor, F. Lanni, “Enhancement of axial resolution in fluorescence microscopy by standing-wave excitation,” Nature 366, 44–48 (1993). [CrossRef] [PubMed]
  12. I. Tasaki, T. Nakaye, “Rapid mechanical responses of the dark-adapted squid retina to light pulses,” Science 223, 411–413 (1984). [CrossRef] [PubMed]
  13. R. D. Allen, J. Metuzals, I. Tasaki, S. T. Brady, S. P. Gilbert, “Fast axonal transport in squid giant axon,” Science 218, 1127–1129 (1982). [CrossRef] [PubMed]
  14. G. C. Bonazzola, A. DeMarco, M. Maringelli, S. Rinaudo, A. Trabucco, “Possible application of the optical tunnel effect to membrane biophysics,” Eur. Biophys. J. 12, 51–55 (1985). [CrossRef] [PubMed]
  15. P. R. Gordon-Weeks, “The Ultrastructure of the neuronal growth cone: new insights from subcellular fractionation and rapid freezing studies,” Electron Microsci. Rev. 1, 201–219 (1988). [CrossRef]
  16. P. R. Gordon-Weeks, “Growth cones—the mechanism of neurite advance,” Bioessays 13, 235–239 (1991). [CrossRef] [PubMed]
  17. J. Q. Zheng, J. J. Wan, M. M. Poo, “Essential role of filopodia in chemotropic turning of nerve growth cone induced by a glutamate gradient,” J. Neurosci. 16, 1140–1149 (1994).
  18. J. Hwang, L. K. Tamm, C. Bohm, T. S. Ramalingam, E. Betzig, M. Edidin, “Nanoscale complexity of phospholipid monolayers investigated by near-field scanning optical microscopy,” Science 270, 610–614 (1995). [CrossRef] [PubMed]
  19. M. Courtois, S. Khatami, E. Fantini, P. Athias, P. Mielle, A. Grynberg, “Polyunsaturated fatty acids in cultured cardiomyocytes: effect on physiology and b-adrenoceptor function,” Am. J. Physiol. 262, H451–H456 (1992). [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited