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Applied Optics

Applied Optics


  • Vol. 38, Iss. 31 — Nov. 1, 1999
  • pp: 6673–6676

Influence of saline media on the fluorescence emission of Bacillus spores

Glenn O. Rubel and K. Hang Fung  »View Author Affiliations

Applied Optics, Vol. 38, Issue 31, pp. 6673-6676 (1999)

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Single-particle levitation in conjunction with 264.3-nm laser excitation is used to measure the fluorescence emission of individual particles of Bacillus globigii spores. With precise humidity control, the fluorescence emission of wetted and desiccated Bacillus spore particles is measured from 300 to 450 nm. Comparison of spectra for Bacillus spores suspended in a standard buffer aqueous solution and for a desiccated 10-µm-diameter aggregate Bacillus spore particle shows that the spectra is virtually indistinguishable. However, at 85% relative humidity, corresponding to a 4.5M sodium chloride solution, the spore spectra redshifts by approximately 25 nm. It is postulated that the spectra redshifting is a result of specific interactions between the tyrosine fluorophore of the Bacillus spore and the phosphate moieties in the buffer solution.

© 1999 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

Original Manuscript: March 30, 1999
Revised Manuscript: July 2, 1999
Published: November 1, 1999

Glenn O. Rubel and K. Hang Fung, "Influence of saline media on the fluorescence emission of Bacillus spores," Appl. Opt. 38, 6673-6676 (1999)

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