We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation. For phase-contrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system.
© 1999 Optical Society of America
(040.1520) Detectors : CCD, charge-coupled device
(090.0090) Holography : Holography
(090.1000) Holography : Aberration compensation
(110.0180) Imaging systems : Microscopy
(350.5030) Other areas of optics : Phase
Etienne Cuche, Pierre Marquet, and Christian Depeursinge, "Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms," Appl. Opt. 38, 6994-7001 (1999)