OSA's Digital Library

Applied Optics

Applied Optics


  • Vol. 38, Iss. 34 — Dec. 1, 1999
  • pp: 7085–7094

Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence

Frank Dubois, Luc Joannes, and Jean-Claude Legros  »View Author Affiliations

Applied Optics, Vol. 38, Issue 34, pp. 7085-7094 (1999)

View Full Text Article

Enhanced HTML    Acrobat PDF (1544 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A digital holographic technique is implemented in a microscope for three-dimensional imaging reconstruction. The setup is a Mach–Zehnder interferometer that uses an incoherent light source to remove the coherent noise that is inherent in the laser sources. A phase-stepping technique determines the optical phase in the image plane of the microscope. Out-of-focus planes are refocused by digital holographic computations, thus considerably enlarging the depth of investigation without the need to change the optical focus mechanically. The technique can be implemented in transmission for various magnification ratios and can cover a wide range of applications. Performances and limitations of the microscope are theoretically evaluated. Experimental results for a test target are given, and examples of two applications in particle localization and investigation of biological sample are provided.

© 1999 Optical Society of America

OCIS Codes
(030.1640) Coherence and statistical optics : Coherence
(030.1670) Coherence and statistical optics : Coherent optical effects
(090.0090) Holography : Holography
(090.1760) Holography : Computer holography
(170.0180) Medical optics and biotechnology : Microscopy
(180.3170) Microscopy : Interference microscopy

Original Manuscript: May 27, 1999
Revised Manuscript: August 16, 1999
Published: December 1, 1999

Frank Dubois, Luc Joannes, and Jean-Claude Legros, "Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence," Appl. Opt. 38, 7085-7094 (1999)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. D. Gabor, “A new microscope principle,” Nature (London) 161, 777–778 (1948). [CrossRef]
  2. U. Schnars, W. Jüptner, “Direct recording of holograms by a CCD target and numeral reconstruction,” Appl. Opt. 33, 179–181 (1994). [CrossRef] [PubMed]
  3. T. M. Kreis, W. P. O. Jüptner, “Principle of digital holography,” in Proceedings of the Third International Workshop on Automatic Processing of Fringe Patterns (Fringe ’97), Vol. 3 of the Akademie Verlag Series in Optical Metrology (Akademie Verlag, Berlin, 1997), pp. 353–363.
  4. B. Nilsson, T. E. Carlsson, “Direct three-dimensional shape measurement by digital light-in-flight holography,” Appl. Opt. 37, 7954–7959 (1998). [CrossRef]
  5. E. Cuche, F. Bevilacqua, C. Depeursinge, “Digital holography for quantitative phase contrast imaging,” Opt. Lett. 24, 291–293 (1999). [CrossRef]
  6. I. Yamaguchi, T. Zhang, “Phase-shifting digital holography,” Opt. Lett. 22, 1268–1270 (1997). [CrossRef] [PubMed]
  7. T. Zhang, I. Yamaguchi, “Three-dimensional microscopy with phase-shifting digital holography,” Opt. Lett. 23, 1221–1223 (1998). [CrossRef]
  8. D. O. Hogenboom, C. A. Dimarzio, T. J. Gaudette, A. J. Devaney, S. C. Lindberg, “Three-dimensional images generated by quadrature interferometry,” Opt. Lett. 23, 783–785 (1998). [CrossRef]
  9. M. Adams, T. M. Kreis, W. P. O. Jüptner, “Particle size and position measurement with digital holography,” in Optical Inspection and Micromeasurements, C. Gorecki, ed., Proc. SPIE3098, 234–240 (1998). [CrossRef]
  10. P. Chavel, “Optical noise and temporal coherence,” J. Opt. Soc. Am. 70, 935–943 (1980). [CrossRef]
  11. M. Nazarathy, J. Shamir, “Fourier optics described by operator algebra,” J. Opt. Soc. Am. 70, 150–159 (1980). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited