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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 38, Iss. 34 — Dec. 1, 1999
  • pp: 7085–7094

Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence

Frank Dubois, Luc Joannes, and Jean-Claude Legros  »View Author Affiliations


Applied Optics, Vol. 38, Issue 34, pp. 7085-7094 (1999)
http://dx.doi.org/10.1364/AO.38.007085


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Abstract

A digital holographic technique is implemented in a microscope for three-dimensional imaging reconstruction. The setup is a Mach–Zehnder interferometer that uses an incoherent light source to remove the coherent noise that is inherent in the laser sources. A phase-stepping technique determines the optical phase in the image plane of the microscope. Out-of-focus planes are refocused by digital holographic computations, thus considerably enlarging the depth of investigation without the need to change the optical focus mechanically. The technique can be implemented in transmission for various magnification ratios and can cover a wide range of applications. Performances and limitations of the microscope are theoretically evaluated. Experimental results for a test target are given, and examples of two applications in particle localization and investigation of biological sample are provided.

© 1999 Optical Society of America

OCIS Codes
(030.1640) Coherence and statistical optics : Coherence
(030.1670) Coherence and statistical optics : Coherent optical effects
(090.0090) Holography : Holography
(090.1760) Holography : Computer holography
(170.0180) Medical optics and biotechnology : Microscopy
(180.3170) Microscopy : Interference microscopy

History
Original Manuscript: May 27, 1999
Revised Manuscript: August 16, 1999
Published: December 1, 1999

Citation
Frank Dubois, Luc Joannes, and Jean-Claude Legros, "Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence," Appl. Opt. 38, 7085-7094 (1999)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-38-34-7085

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