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Applied Optics

Applied Optics


  • Vol. 38, Iss. 36 — Dec. 20, 1999
  • pp: 7393–7397

Third-harmonic generation microscopy by use of a compact, femtosecond fiber laser source

Andrew C. Millard, Paul W. Wiseman, David N. Fittinghoff, Kent R. Wilson, Jeffrey A. Squier, and Michiel Müller  »View Author Affiliations

Applied Optics, Vol. 38, Issue 36, pp. 7393-7397 (1999)

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We demonstrate the first use, to our knowledge, of a compact, diode-pumped, femtosecond fiber laser for third-harmonic generation (THG) microscopy. We discuss the utility of this technique, as well as the technical issues involved in using this compact source, and demonstrate the first use, to our knowledge, of imaging by THG backlighting.

© 1999 Optical Society of America

OCIS Codes
(140.3510) Lasers and laser optics : Lasers, fiber
(140.4050) Lasers and laser optics : Mode-locked lasers
(140.7090) Lasers and laser optics : Ultrafast lasers
(180.0180) Microscopy : Microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(320.7110) Ultrafast optics : Ultrafast nonlinear optics

Original Manuscript: April 30, 1999
Revised Manuscript: August 25, 1999
Published: December 20, 1999

Andrew C. Millard, Paul W. Wiseman, David N. Fittinghoff, Kent R. Wilson, Jeffrey A. Squier, and Michiel Müller, "Third-harmonic generation microscopy by use of a compact, femtosecond fiber laser source," Appl. Opt. 38, 7393-7397 (1999)

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  1. J. A. Squier, M. Müller, G. J. Brakenhoff, K. R. Wilson, “Third harmonic generation microscopy,” Opt. Exp. 3, 315–324 (1998). [CrossRef]
  2. Y. Barad, H. Eisenberg, M. Horowitz, Y. Silberberg, “Non-linear scanning laser microscopy by third harmonic generation,” Appl. Phys. Lett. 70, 922–924 (1997). [CrossRef]
  3. M. Müller, J. A. Squier, K. R. Wilson, G. J. Brakenhoff, “3D microscopy of transparent objects using third harmonic generation,” J. Microsc. 191, 266–274 (1998). [CrossRef] [PubMed]
  4. J. A. Squier, M. Müller, “Third-harmonic generation imaging of laser-induced breakdown in glass,” Appl. Opt. 38, 5789–5794 (1999). [CrossRef]
  5. T. Y. F. Tsang, “Optical third harmonic generation at interfaces,” Phys. Rev. A 52, 4116–4125 (1995). [CrossRef] [PubMed]
  6. T. Y. F. Tsang, “Reflected optical harmonics from dielectric mirrors,” Appl. Opt. 33, 7720–7724 (1994). [CrossRef] [PubMed]
  7. D. Yelin, Y. Silberberg, Y. Barad, J. S. Patel, “Phase-matched third harmonic generation in a nematic liquid crystal,” Phys. Rev. Lett. 82, 3046–3049 (1999). [CrossRef]
  8. C. Preza, “Phase estimation using rotational diversity for differential interference contrast microscopy,” D. S. thesis (Department of Electrical Engineering, University of Washington, Saint Louis, Mo., 1998).
  9. M. E. Fermann, L.-M. Yang, M. L. Stock, M. T. Andrejco, “Environmentally stable Kerr-type mode-locked erbium fiber laser producing 360-fs pulses,” Opt. Lett. 19, 43–45 (1994). [CrossRef] [PubMed]
  10. R. Trebino, K. W. DeLong, D. N. Fittinghoff, J. N. Sweetser, M. A. Krumbügel, B. A. Richman, D. J. Kane, “Measuring ultrashort laser pulses in the time-frequency domain using frequency-resolved optical gating,” Rev. Sci. Instrum. 68, 3277–3295 (1997). [CrossRef]
  11. J. A. Curcio, C. C. Petty, “The near infrared absorption spectrum of liquid water,” J. Opt. Soc. Am. 41, 302–304 (1951). [CrossRef]
  12. A. Schönle, S. W. Hell, “Heating by absorption in the focus of an objective lens,” Opt. Lett. 23, 325–327 (1998). [CrossRef]
  13. D. Kleinfeld, P. P. Mitra, F. Helmchen, W. Denk, “Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex,” Proc. Natl. Acad. Sci. USA 95, 15,741–15,746 (1998). [CrossRef]

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