We report on three-dimensional autofluorescence spectroscopy obtained from rat skeletal muscle tissue under two-photon excitation by an ultrashort pulsed-laser beam. It is demonstrated that two types of fluorophores within the skeletal muscle tissue can be simultaneously excited with the laser beam at a wavelength of 800 nm. The two fluorophores exhibited unique fluorescence spectral peaks at wavelengths of 450 and 550 nm. These spectroscopic signals can be used to form a three-dimensional image, giving the information about the biochemical makeup of the skeletal muscle tissue.
© 1999 Optical Society of America
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6930) Medical optics and biotechnology : Tissue
Steven P. Schilders and Min Gu, "Three-Dimensional Autofluorescence Spectroscopy of Rat Skeletal Muscle Tissue Under Two-Photon Excitation," Appl. Opt. 38, 720-723 (1999)