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Applied Optics

Applied Optics


  • Vol. 38, Iss. 4 — Feb. 1, 1999
  • pp: 720–723

Three-dimensional autofluorescence spectroscopy of rat skeletal muscle tissue under two-photon excitation

Steven P. Schilders and Min Gu  »View Author Affiliations

Applied Optics, Vol. 38, Issue 4, pp. 720-723 (1999)

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We report on three-dimensional autofluorescence spectroscopy obtained from rat skeletal muscle tissue under two-photon excitation by an ultrashort pulsed-laser beam. It is demonstrated that two types of fluorophores within the skeletal muscle tissue can be simultaneously excited with the laser beam at a wavelength of 800 nm. The two fluorophores exhibited unique fluorescence spectral peaks at wavelengths of 450 and 550 nm. These spectroscopic signals can be used to form a three-dimensional image, giving the information about the biochemical makeup of the skeletal muscle tissue.

© 1999 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6930) Medical optics and biotechnology : Tissue

Original Manuscript: June 18, 1998
Revised Manuscript: September 30, 1998
Published: February 1, 1999

Steven P. Schilders and Min Gu, "Three-dimensional autofluorescence spectroscopy of rat skeletal muscle tissue under two-photon excitation," Appl. Opt. 38, 720-723 (1999)

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  1. B. Chance, P. Cohen, F. Jobsis, B. Schoener, “Intracellular oxidation states in vivo,” Science 137, 499–508 (1962). [CrossRef] [PubMed]
  2. J. Aubin, “Autofluorescence of viable cultured mammalian cells,” J. Histochem. Cytochem. 27, 36–43 (1979). [CrossRef] [PubMed]
  3. R. C. Benson, R. A. Meyer, M. E. Zauba, G. M. McKhann, “Cellular autofluorescence—is it due to flavins?” J. Histochem. Cytochem. 27, 44–48 (1979). [CrossRef] [PubMed]
  4. R. R. Alfano, D. B. Tata, J. Cordero, P. Tomashefsky, F. W. Longo, M. A. Alfano, “Laser induced fluorescence spectroscopy from native cancerous and normal tissue,” IEEE J. Quantum Electron. QE-20, 1507–1511 (1984). [CrossRef]
  5. G. C. Tang, A. Pradhan, R. R. Alfano, “Spectroscopic differences between human cancer and normal lung and breast tissues,” Lasers Surg. Med. 9, 290–295 (1989). [CrossRef] [PubMed]
  6. W. Denk, J. H. Strickler, W. W. Webb, “Two photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990). [CrossRef] [PubMed]
  7. Y. Guo, Q. Z. Wang, N. Zhadin, F. Liu, S. Demos, D. Calistru, A. Tirksliunas, A. Katz, Y. Bunansky, P. P. Ho, R. R. Alfano, “Two-photon excitation of fluorescence from chicken tissue,” Appl. Opt. 36, 968–970 (1997). [CrossRef] [PubMed]
  8. C. J. R. Sheppard, M. Gu, “Image formation in two-photon fluorescence microscopy,” Optik 86, 104–106 (1990).
  9. M. Gu, Principles of Three-Dimensional Imaging in Confocal Microscopes (World Scientific, Singapore, 1996).
  10. B. R. Masters, P. T. C. So, E. Gratton, “Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin,” Biophys. J. 72, 2405–2412 (1997). [CrossRef] [PubMed]
  11. W. J. Krause, Essentials of Human Histology, (Little, Brown and Company, Boston, Mass., 1994).
  12. Y. R. Shen, Principles of Nonlinear Optics (Wiley, New York, 1984).

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