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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 38, Iss. 4 — Feb. 1, 1999
  • pp: 720–723

Three-dimensional autofluorescence spectroscopy of rat skeletal muscle tissue under two-photon excitation

Steven P. Schilders and Min Gu  »View Author Affiliations


Applied Optics, Vol. 38, Issue 4, pp. 720-723 (1999)
http://dx.doi.org/10.1364/AO.38.000720


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Abstract

We report on three-dimensional autofluorescence spectroscopy obtained from rat skeletal muscle tissue under two-photon excitation by an ultrashort pulsed-laser beam. It is demonstrated that two types of fluorophores within the skeletal muscle tissue can be simultaneously excited with the laser beam at a wavelength of 800 nm. The two fluorophores exhibited unique fluorescence spectral peaks at wavelengths of 450 and 550 nm. These spectroscopic signals can be used to form a three-dimensional image, giving the information about the biochemical makeup of the skeletal muscle tissue.

© 1999 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6930) Medical optics and biotechnology : Tissue

History
Original Manuscript: June 18, 1998
Revised Manuscript: September 30, 1998
Published: February 1, 1999

Citation
Steven P. Schilders and Min Gu, "Three-dimensional autofluorescence spectroscopy of rat skeletal muscle tissue under two-photon excitation," Appl. Opt. 38, 720-723 (1999)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-38-4-720


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