Three-dimensional fluorescence spatial distributions under single-photon and two-photon excitation within a turbid medium are studied with Monte Carlo simulation. It is demonstrated that two-photon excitation has an advantage of producing much less fluorescence light outside the focal region compared with single-photon excitation. With the increase of the concentration of scattering particles in a turbid medium, the position of the maximum fluorescence intensity point shifts from the geometric focal region toward the medium surface. Further studies show that the optical sectioning property of two-photon fluorescence microscopy is degraded in thick turbid media or when the numerical aperture of an objective becomes low.

© 2000 Optical Society of America

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Related Journal Articles

• Rapid modeling of diffuse reflectance of light in turbid slabs (JOSAA)
• Nonuniqueness in optical tomography: relevance of the P1 approximation (OL)
• Spatial Distribution of Two-Photon-Excited Fluorescence in Scattering Media (AO)
• Spatial distribution of two-photon-excited fluorescence in scattering media: erratum (AO)
• Inclusion Characterization in a Scattering Slab with Time-Resolved Transmittance Measurements: Perturbation Analysis (AO)

Related Conference Papers

• Ballistic imaging in the liquid core of a spray
• Two-Photon Absorption Fluorescence Imaging to Characterize Microfluidic Device Performance
• All-Chirped-Mirror Pulse Compressor for Nonlinear Microscopy
• Field Pattern of Metal Hole-Arrays Opens the Way for a New Near- and Mid-Field High Resolution Microscopy
• Optical Rotation Detection in a Chiral Scattering Medium with an Amplitude Sensitive Balanced Detection Polarimeter