OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 10 — Apr. 1, 2000
  • pp: 1575–1579

Spatial distribution of single-photon and two-photon fluorescence light in scattering media: Monte Carlo simulation

Xiaosong Gan and Min Gu  »View Author Affiliations


Applied Optics, Vol. 39, Issue 10, pp. 1575-1579 (2000)
http://dx.doi.org/10.1364/AO.39.001575


View Full Text Article

Enhanced HTML    Acrobat PDF (249 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Three-dimensional fluorescence spatial distributions under single-photon and two-photon excitation within a turbid medium are studied with Monte Carlo simulation. It is demonstrated that two-photon excitation has an advantage of producing much less fluorescence light outside the focal region compared with single-photon excitation. With the increase of the concentration of scattering particles in a turbid medium, the position of the maximum fluorescence intensity point shifts from the geometric focal region toward the medium surface. Further studies show that the optical sectioning property of two-photon fluorescence microscopy is degraded in thick turbid media or when the numerical aperture of an objective becomes low.

© 2000 Optical Society of America

OCIS Codes
(110.7050) Imaging systems : Turbid media
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(290.7050) Scattering : Turbid media

History
Original Manuscript: May 10, 1999
Revised Manuscript: August 26, 1999
Published: April 1, 2000

Citation
Xiaosong Gan and Min Gu, "Spatial distribution of single-photon and two-photon fluorescence light in scattering media: Monte Carlo simulation," Appl. Opt. 39, 1575-1579 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-10-1575

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited