Digital holograms are recorded of biological tissues by use of a Q-switched double-pulsed ruby laser. An image-plane digital holography setup is used with a CCD camera for capturing two holograms with a short time separation (20–800 µs). Subtraction of the phase distribution in two digital holograms yield a fringe phase map that shows the change in deformation of the tissue surface between the recordings. Experiments are performed on tissue from a pig that was excited by a short-shock pulse and on a human hand that was excited by sinusoidal stimulation. Results when the object is imaged through an endoscope are also presented. The technique could be an approach for measuring parameters like elasticity on biological tissues.
© 2000 Optical Society of America
Original Manuscript: December 1, 1999
Revised Manuscript: February 23, 2000
Published: June 1, 2000
Staffan Schedin, Giancarlo Pedrini, and Hans J. Tiziani, "Pulsed digital holography for deformation measurements on biological tissues," Appl. Opt. 39, 2853-2857 (2000)