Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Excitation-and-collection geometry insensitive fluorescence imaging of tissue-simulating turbid media

Not Accessible

Your library or personal account may give you access

Abstract

We present an imaging technique for the correction of geometrical effects in fluorescence measurement of optically thick, turbid media such as human tissue. Specifically, we use the cross-polarization method to reject specular reflection and enhance the diffusive backscattering of polarized fluorescence excitation light from the turbid media. We correct the nonuniformity of the image field caused by the excitation-and-collection geometry of a fluorescence imaging system by normalizing the fluorescence image to the cross-polarized reflection image. The ratio image provides a map of relative fluorescence yield, defined as the ratio of emerging fluorescence power to incident excitation, over the surface of an imaged homogeneous turbid medium when fluorescence excitation-and-collection geometries vary in a wide range. We investigate the mechanism of ratio imaging by using Monte Carlo modeling. Our findings show that this technique could have a potential use in the detection of early cancer, which usually starts from a superficial layer of tissue, based on the contrast in the tissue fluorescence of an early lesion and of the surrounding normal tissue.

© 2000 Optical Society of America

Full Article  |  PDF Article
More Like This
Preliminary study of detecting neoplastic growths in vivo with real time calibrated autofluorescence imaging

Tao Wu, Jianan Y. Qu, Tak-Hong Cheung, Keith Wing-Kit Lo, and Mei-Yung Yu
Opt. Express 11(4) 291-298 (2003)

Experimental and simulated angular profiles of fluorescence and diffuse reflectance emission from turbid media

Steven C. Gebhart, Anita Mahadevan-Jansen, and Wei-Chiang Lin
Appl. Opt. 44(23) 4884-4901 (2005)

Influence of the emission–reception geometry in laser-induced fluorescence spectra from turbid media

Sigrid Avrillier, Eric Tinet, Dominique Ettori, Jean-Michel Tualle, and Bernard Gélébart
Appl. Opt. 37(13) 2781-2787 (1998)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (14)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved