We have constructed tapered fiber-optic microsensors with a tip diameter of less than 10 μm to measure profiles of backscattered light in biofilms, which are thin layers of micro-organisms firmly attached to surfaces. The observed response agrees well with local effective diffusivity microelectrode measurements, with <i>R</i><sup>2</sup> > 0.85. A strong relation between signal intensity and wavelength has been observed at 670 and 1320 nm. These sensors have the potential to replace local effective diffusivity microelectrodes for true <i>in situ</i> biofilm measurements.
© 2000 Optical Society of America
Haluk Beyenal, Zbigniew Lewandowski, Chris Yakymyshyn, Brad Lemley, and Joe Wehri, "Fiber-Optic Microsensors to Measure Backscattered Light Intensity in Biofilms," Appl. Opt. 39, 3408-3412 (2000)