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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 22 — Aug. 1, 2000
  • pp: 3945–3947

Reduced effects of spherical aberration on penetration depth under two-photon excitation

Djenan Ganic, Xiaosong Gan, and Min Gu  »View Author Affiliations


Applied Optics, Vol. 39, Issue 22, pp. 3945-3947 (2000)
http://dx.doi.org/10.1364/AO.39.003945


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Abstract

We compare the effects of spherical aberration on the penetration depth of single-photon and two-photon excitation for instances in which the aberration is caused by the refractive-index mismatch when a beam is focused through an interface. It is shown both theoretically and experimentally that two-photon fluorescence imaging experiences less spherical aberration and can thus propagate to a deeper depth within a thick medium.

© 2000 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

History
Original Manuscript: February 4, 2000
Revised Manuscript: May 9, 2000
Published: August 1, 2000

Citation
Djenan Ganic, Xiaosong Gan, and Min Gu, "Reduced effects of spherical aberration on penetration depth under two-photon excitation," Appl. Opt. 39, 3945-3947 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-22-3945


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References

  1. W. Denk, J. H. Strickler, W. W. Webb, “Two photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990). [CrossRef] [PubMed]
  2. P. Török, P. Varga, Z. Laczik, G. R. Booker, “Electromagnetic diffraction of light focused through a planar interface between materials of mismatched refractive indices: an integral representation,” J. Opt. Soc. Am. A 12, 325–332 (1995). [CrossRef]
  3. P. Török, P. Varga, G. R. Booker, “Electromagnetic diffraction of light focused through a planar interface between materials of mismatched refractive indices: structure of the electromagnetic field. I,” J. Opt. Soc. Am. A 12, 2136–2144 (1995). [CrossRef]
  4. P. Török, P. Varga, A. Konkol, G. R. Booker, “Electromagnetic diffraction of light focused through a planar interface between materials of mismatched refractive indices: structure of the electromagnetic field. II,” J. Opt. Soc. Am. A 13, 2232–2238 (1996). [CrossRef]
  5. C. J. R. Sheppard, P. Török, “Effects of specimen refractive index on confocal imaging,” J. Microscopy 185, 366–374 (1997). [CrossRef]
  6. P. D. Higdon, P. Török, T. Wilson, “Imaging properties of high aperture multiphoton fluorescence scanning optical microscopes,” J. Microscopy 193, 127–141 (1999). [CrossRef]
  7. D. Day, M. Gu, “Effects of refractive-index mismatch on three-dimensional optical data storage density in a two-photon bleaching polymer,” Appl. Opt. 37, 6299–6304 (1998). [CrossRef]
  8. S. Hell, E. H. K. Stelzer, “Properties of a 4Pi confocal fluorescence microscope,” J. Opt. Soc. Am. A 9, 2159–2166 (1992). [CrossRef]
  9. M. Gu, Principles of Three-Dimensional Imaging in Confocal Microscopes (World Scientific, Singapore, 1996).

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