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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 22 — Aug. 1, 2000
  • pp: 3945–3947

Reduced effects of spherical aberration on penetration depth under two-photon excitation

Djenan Ganic, Xiaosong Gan, and Min Gu  »View Author Affiliations


Applied Optics, Vol. 39, Issue 22, pp. 3945-3947 (2000)
http://dx.doi.org/10.1364/AO.39.003945


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Abstract

We compare the effects of spherical aberration on the penetration depth of single-photon and two-photon excitation for instances in which the aberration is caused by the refractive-index mismatch when a beam is focused through an interface. It is shown both theoretically and experimentally that two-photon fluorescence imaging experiences less spherical aberration and can thus propagate to a deeper depth within a thick medium.

© 2000 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

History
Original Manuscript: February 4, 2000
Revised Manuscript: May 9, 2000
Published: August 1, 2000

Citation
Djenan Ganic, Xiaosong Gan, and Min Gu, "Reduced effects of spherical aberration on penetration depth under two-photon excitation," Appl. Opt. 39, 3945-3947 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-22-3945

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