OSA's Digital Library

Applied Optics

Applied Optics


  • Vol. 39, Iss. 24 — Aug. 20, 2000
  • pp: 4431–4435

Changes in porphyrin nonlinear absorption owing to interaction with bovine serum albumin

Iouri E. Borissevitch, Nikifor Rakov, Glauco S. Maciel, and Cid B. de Araújo  »View Author Affiliations

Applied Optics, Vol. 39, Issue 24, pp. 4431-4435 (2000)

View Full Text Article

Enhanced HTML    Acrobat PDF (101 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



The changes in the nonlinear absorption of meso-tetrakis (p-sulfonatophenyl) porphyrin (TPPS4) caused by its binding to bovine serum albumin (BSA) were investigated in aqueous solutions. The nonlinear absorption depended on the excitation wavelength and pH. It is noteworthy that, for excitation at 640 nm, saturated absorption was observed in a TPPS4 solution (pH 4.0), whereas reverse saturated absorption occurred for all pH values from 4.0 to 7.0 when BSA was added to TPPS4. The presence of BSA also initiates an increase in the saturation intensity and a reduction of the nonlinear absorption coefficient. The interpretation of the results based on the binding of TPPS4 to BSA is corroborated by linear absorption, fluorescence, light scattering, and flash-photolysis data.

© 2000 Optical Society of America

OCIS Codes
(160.4890) Materials : Organic materials
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials
(300.6460) Spectroscopy : Spectroscopy, saturation

Original Manuscript: September 19, 1999
Revised Manuscript: April 21, 2000
Published: August 20, 2000

Iouri E. Borissevitch, Nikifor Rakov, Glauco S. Maciel, and Cid B. de Araújo, "Changes in porphyrin nonlinear absorption owing to interaction with bovine serum albumin," Appl. Opt. 39, 4431-4435 (2000)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. W. Blau, H. Byrne, W. M. Dennis, J. M. Kelly, “Reverse saturable absorption in tetraphenyl porphyrins,” Opt. Commun. 56, 25–29 (1985). [CrossRef]
  2. S. Guha, K. Kang, P. Porter, J. F. Roach, D. E. Remy, F. J. Aranda, D. V. G. L. N. Rao, “Third-order optical nonlinearities of metallotetrabenzoporphyrins and platinum polyyne,” Opt. Lett. 17, 264–266 (1992). [CrossRef] [PubMed]
  3. G. L. Wood, M. J. Miller, A. G. Mott, “Investigation of tetrabenzoporphyrin by the Z-scan technique,” Opt. Lett. 20, 973–975 (1995). [CrossRef]
  4. K. Kandasamy, P. N. Puntambekar, B. P. Singh, S. J. Shetty, T. S. Srivastava, “Resonant nonlinear optical studies on porphyrin derivatives,” J. Nonlin. Opt. Phys. Mater. 6, 361–375 (1997). [CrossRef]
  5. W. Sun, C. C. Byeon, M. M. McKerns, C. M. Lawson, G. M. Gray, D. Wang, “Optical limiting performances of asymmetric pentaazodentate porphyrin-like cadmium complexes,” Appl. Phys. Lett. 73, 1167–1169 (1998). [CrossRef]
  6. S. R. Mishra, H. S. Rawat, M. Laghate, “Nonlinear absorption and optical limiting in metalloporphyrins,” Opt. Commun. 147, 328–332 (1998). [CrossRef]
  7. K. Dou, X. Sun, X. Wang, R. Parkhill, Y. Guo, E. T. Knobbe, “Optical limiting and upconverted luminescence in metalloporphyrin-doped sol-gels,” Solid State Commun. 107, 101–106 (1998). [CrossRef]
  8. D. Dolphin, ed., The Porphyrins (Academic, New York, 1978), Vols. I–V.
  9. R. Bonnett, “Photosensitizers of the porphyrin and phtalocyanine series for photodynamic therapy,” Chem. Soc. Rev. 24, 19–33 (1995). [CrossRef]
  10. M. Ochsner, “Photochemical and photobiological processes in the photodynamic therapy of tumours,” J. Photochem. Photobiol. B 39, 1–18 (1997). [CrossRef] [PubMed]
  11. I. E. Borissevitch, T. T. Tominaga, H. Imasato, M. Tabak, “Fluorescence and optical absorption study of interaction of two water soluble porphyrins with bovine serum albumin. The role of albumin and porphyrin aggregation,” J. Lumin. 69, 65–76 (1996). [CrossRef]
  12. I. E. Borissevitch, T. T. Tominaga, H. Imasato, M. Tabak, “Resonance light scattering study of aggregation of two water soluble porphyrins due to their interaction with bovine serum albumin,” Anal. Chim. Acta 343, 281–286 (1997). [CrossRef]
  13. S. C. Monte, I. E. Borissevitch, J. R. Perussi, H. Imasato, M. Tabak, “Aggregation of meso-tetrakis (4-N-methyl-pyridinimil) porphyrin in its free base, Fe(III) and Mn(III) forms due to the interaction with DNA in aqueous solution,” J. Lumin. 78, 53–61 (1998). [CrossRef]
  14. I. E. Borissevitch, T. T. Tominaga, C. C. Schmitt, “Photophysical studies on the interaction of two water-soluble porphyrins with bovine serum albumin. Effects upon the porphyrin triplet state characteristics,” J. Photochem. Photobiol. A 114, 201–207 (1998). [CrossRef]
  15. I. E. Borissevitch, S. C. M. Gandini, “Photophysical studies of excited state characteristics of meso-tetrakis(4-N-methyl-pyridiniumyl) porphyrin bound to DNA,” J. Photochem. Photobiol. B 43/2, 112–120 (1998). [CrossRef]
  16. S. C. M. Gandini, V. E. Yushmanov, I. E. Borissevitch, M. Tabak, “Interaction of the tetra (4-sulfonatophenyl) porphyrin with ionic surfactants: aggregation and location in micelles,” Langmuir 15, 6233–6243 (1999). [CrossRef]
  17. V. E. Yushmanov, H. Imasato, T. T. Tominaga, M. Tabak, “1H NMR and electronic absorption spectroscopy of paramagnetic water-soluble meso-tetraarylsubstituted cationic and anionic metalloporphyrins,” J. Inorg. Biochem. 61, 233–241 (1996). [CrossRef] [PubMed]
  18. G. G. Hammer, ed., Investigation of Rates and Mechanisms of Reactions (Wiley, New York, 1974).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited