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Applied Optics

Applied Optics


  • Vol. 39, Iss. 25 — Sep. 1, 2000
  • pp: 4733–4745

Quantifying the properties of two-layer turbid media with frequency-domain diffuse reflectance

Tuan H. Pham, Thorsten Spott, Lars O. Svaasand, and Bruce J. Tromberg  »View Author Affiliations

Applied Optics, Vol. 39, Issue 25, pp. 4733-4745 (2000)

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Noncontact, frequency-domain measurements of diffusely reflected light are used to quantify optical properties of two-layer tissuelike turbid media. The irradiating source is a sinusoidal intensity-modulated plane wave, with modulation frequencies ranging from 10 to 1500 MHz. Frequency-dependent phase and amplitude of diffusely reflected photon density waves are simultaneously fitted to a diffusion-based two-layer model to quantify absorption (μ a ) and reduced scattering (μ s ′) parameters of each layer as well as the upper-layer thickness (l). Study results indicate that the optical properties of two-layer media can be determined with a percent accuracy of the order of ±9% and ±5% for μ a and μ s ′, respectively. The accuracy of upper-layer thickness (l) estimation is as good as ±6% when optical properties of upper and lower layers are known. Optical property and layer thickness prediction accuracy degrade significantly when more than three free parameters are extracted from data fits. Problems with convergence are encountered when all five free parameters (μ a and μ s ′ of upper and lower layers and thickness l) must be deduced.

© 2000 Optical Society of America

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.5270) Medical optics and biotechnology : Photon density waves
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

Original Manuscript: December 10, 1999
Revised Manuscript: May 19, 2000
Published: September 1, 2000

Tuan H. Pham, Thorsten Spott, Lars O. Svaasand, and Bruce J. Tromberg, "Quantifying the properties of two-layer turbid media with frequency-domain diffuse reflectance," Appl. Opt. 39, 4733-4745 (2000)

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