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Applied Optics

Applied Optics


  • Vol. 39, Iss. 28 — Oct. 1, 2000
  • pp: 5231–5243

Noninvasive investigation of blood oxygenation dynamics of tumors by near-infrared spectroscopy

Hanli Liu, Yulin Song, Katherine L. Worden, Xin Jiang, Anca Constantinescu, and Ralph P. Mason  »View Author Affiliations

Applied Optics, Vol. 39, Issue 28, pp. 5231-5243 (2000)

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The measurement of dynamic changes in the blood oxygenation of tumor vasculature could be valuable for tumor prognosis and optimizing tumor treatment plans. In this study we employed near-infrared spectroscopy (NIRS) to measure changes in the total hemoglobin concentration together with the degree of hemoglobin oxygenation in the vascular bed of breast and prostate tumors implanted in rats. Measurements were made while inhaled gas was alternated between 33% oxygen and carbogen (95% O2, 5% CO2). Significant dynamic changes in tumor oxygenation were observed to accompany respiratory challenge, and these changes could be modeled with two exponential components, yielding two time constants. Following the Fick principle, we derived a simplified model to relate the time constants to tumor blood-perfusion rates. This study demonstrates that the NIRS technology can provide an efficient, real-time, noninvasive means of monitoring the vascular oxygenation dynamics of tumors and facilitate investigations of tumor vascular perfusion. This may have prognostic value and promises insight into tumor vascular development.

© 2000 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.5280) Medical optics and biotechnology : Photon migration
(290.1990) Scattering : Diffusion
(290.7050) Scattering : Turbid media

Original Manuscript: March 16, 2000
Revised Manuscript: June 25, 2000
Published: October 1, 2000

Hanli Liu, Yulin Song, Katherine L. Worden, Xin Jiang, Anca Constantinescu, and Ralph P. Mason, "Noninvasive investigation of blood oxygenation dynamics of tumors by near-infrared spectroscopy," Appl. Opt. 39, 5231-5243 (2000)

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