OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 33 — Nov. 20, 2000
  • pp: 6257–6262

Thermal-lens-induced anomalous solvent’s effect on fluorescence produced by two-photon continuous-wave laser excitation

Marc Fischer and Chieu D. Tran  »View Author Affiliations


Applied Optics, Vol. 39, Issue 33, pp. 6257-6262 (2000)
http://dx.doi.org/10.1364/AO.39.006257


View Full Text Article

Enhanced HTML    Acrobat PDF (103 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Measurements of two-photon-excited fluorescence (TPF) of fluorescein and Rhodamine 6G in various solvents were performed with a continuous-wave (cw) laser for excitation and an acousto-optic tunable filter for spectral dispersion. Interestingly, the cw laser excitation produced an unwanted thermal-lens effect when the measurements were performed in solvents that absorb the excitation laser light (e.g., alcohols and water, because these solvents absorb the 780-nm excitation light through the overtone and combination transitions of the O—H group). The defocusing effect of the thermal lens leads to a decrease in the TPF signal. Because the strength of the thermal lens depends on the thermo-optical properties (dn/dT and thermal conductivity) of the solvent, its interference makes the effect of solvents on the TPF much different from those on one-photon-excited fluorescence. However, the thermal-lens interference will not limit the application of this cw laser excited TPF technique because, even when measurements were performed in solvents that absorb cw excitation laser light, the thermal-lens interference was observed only in solvents such as nonpolar organic solvents that have relatively better thermo-optical properties. Interference was not observed in water, which is the most widely used solvent for the TPF technique (because water has poor thermo-optical properties).

© 2000 Optical Society of America

OCIS Codes
(230.0230) Optical devices : Optical devices
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6410) Spectroscopy : Spectroscopy, multiphoton
(300.6430) Spectroscopy : Spectroscopy, photothermal

History
Original Manuscript: April 17, 2000
Revised Manuscript: June 30, 2000
Published: November 20, 2000

Citation
Marc Fischer and Chieu D. Tran, "Thermal-lens-induced anomalous solvent’s effect on fluorescence produced by two-photon continuous-wave laser excitation," Appl. Opt. 39, 6257-6262 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-33-6257


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. M. Goeppert-Mayer, “Uber Elementarakte mit zwei Quantensprungen,” Ann. Phys. (Leipzig) 9, 273–295 (1931). [CrossRef]
  2. W. Kaiser, C. G. B. Garrett, “Two photon excitation in CaF2:Eu2+,” Phys. Rev. Lett. 7, 229–231 (1961). [CrossRef]
  3. W. Denk, J. H. Stricker, W. W. Webb, “Two photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990). [CrossRef] [PubMed]
  4. M. J. Wirth, A. C. Koskelo, C. E. Mohler, “Study of solvation symmetry by two photon polarization measurements,” J. Phys. Chem. 87, 4395–4400 (1983). [CrossRef]
  5. C. E. Mohlern, M. J. Wirth, “Solvent perturbations on the excited state symmetry of randomly oriented molecules by two photon absorption,” J. Chem. Phys. 88, 7369–7375 (1988). [CrossRef]
  6. A. Fischer, C. Cremer, E. H. K. Stelzer, “Fluorescence of coumarins and xanthenes after two-photon absorption with a pulsed titanium-sapphire laser,” Appl. Opt. 34, 1989–2003 (1995). [CrossRef] [PubMed]
  7. C. Xu, W. W. Webb, “Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm,” J. Opt. Soc. Am. B 13, 481–491 (1996). [CrossRef]
  8. C. Xu, J. B. Shear, W. W. Webb, “Hyper Rayleigh and hyper Raman scattering background of liquid water in two photon excited fluorescence detection,” Anal. Chem. 69, 1285–1287 (1997). [CrossRef] [PubMed]
  9. C. Radzewicz, P. Glowczewski, J. Krasinski, “High efficient system for studying multiphoton absorption,” Appl. Phys. 17, 423–424 (1978). [CrossRef]
  10. I. M. Catalano, A. Cingolani, “Multiphoton cross-section measurements with low-power cw laser-induced luminescence,” Appl. Opt. 21, 477–480 (1982). [CrossRef] [PubMed]
  11. C. D. Tran, “Acousto-optic devices: new optical elements for spectroscopy,” Anal. Chem. 64, 971A–981A (1992).
  12. C. D. Tran, J. Lu, “Characterization of the acousto-optic tunable filter for the ultraviolet and visible regions and development of an AOTF based rapid scanning detector for HPLC,” Anal. Chim. Acta 314, 57–66 (1995). [CrossRef]
  13. C. D. Tran, “Principles and analytical applications of acousto-optic tunable filter: an overview,” Talanta 45, 237–248 (1997). [CrossRef]
  14. M. S. Baptista, C. D. Tran, “Near-infrared thermal lens spectrometer based on an erbium-doped fiber amplifier and an acousto-optic tunable filter, and its application in the determination of nucleotides,” Appl. Opt. 36, 7059–7065 (1997). [CrossRef]
  15. M. S. Baptista, C. D. Tran, G. H. Gao, “Near infrared detection of flow injection analysis by acousto-optic tunable filter based spectrometry,” Anal. Chem. 68, 971–976 (1996). [CrossRef] [PubMed]
  16. M. Franko, C. D. Tran, “Temperature effect on photothermal lens phenomena in water: photothermal focusing and defocusing,” Chem. Phys. Lett. 158, 31–36 (1989). [CrossRef]
  17. M. Franko, C. D. Tran, “Water as a unique medium for thermal lens measurements,” Anal. Chem. 61, 1660–1666 (1989). [CrossRef]
  18. M. Franko, C. D. Tran, “Thermal lens effect in electrolyte and surfactant media,” J. Phys. Chem. 95, 6688–6696 (1991). [CrossRef]
  19. Fischer Scientific Corporation, Acros Organic Chemicals, 1995/1996 catalog, (FischerScientific, Pittsburgh, Pa., 1995), pp. 878, 1515.
  20. M. Fischer, J. Georges, Spectrochim. Acta Part A 53, 1419–1423 (1997). [CrossRef]
  21. J. P. Hermann, J. Ducuing, “Dispersion of the two photon cross section in rhodamine dyes,” Opt. Commun. 6, 101–105 (1972). [CrossRef]
  22. D. J. Bradley, M. H. R. Hutchinson, T. M. H. Koetser, C. New, M. S. Petty, “Interaction of picosecond laser pulses with organic molecules. I. Two photon fluorescence quenching and singlet states excitation in rhodamine dyes,” Proc. R. Soc. London Ser. A 328, 97–121 (1972). [CrossRef]
  23. K. S. Overway, F. E. Lytle, “Two photon excitation profiles in cylindrical capillaries,” Appl. Spectrosc. 52, 928–932 (1998). [CrossRef]
  24. C. D. Tran, V. I. Grishko, M. S. Baptista, “Nondestructive and nonintrusive determination of chemical and isotopic purity of solvents by near infrared thermal lens spectrometry,” Appl. Spectrosc. 48, 833–842 (1994). [CrossRef]
  25. J. A. Riddick, W. B. Bunger, T. K. Sakano, Organic Solvents—Physical Properties and Methods of Purification (Wiley, New York, 1986).
  26. N. W. Tsederberg, Thermal Conductivity of Gases and Liquids (MIT Press, Cambridge, Mass., 1965).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article

OSA is a member of CrossRef.

CrossCheck Deposited