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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 34 — Dec. 1, 2000
  • pp: 6312–6317

Effect of handling and fixation processes on fluorescence spectroscopy of mouse skeletal muscles under two-photon excitation

Ming G. Xu, Elizabeth D. Williams, Erik W. Thompson, and Min Gu  »View Author Affiliations


Applied Optics, Vol. 39, Issue 34, pp. 6312-6317 (2000)
http://dx.doi.org/10.1364/AO.39.006312


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Abstract

We investigated the effects of handling and fixation processes on the two-photon fluorescence spectroscopy of endogenous fluorophors in mouse skeletal muscle. The skeletal muscle was handled in one of two ways: either sectioned without storage or sectioned following storage in a freezer. The two-photon fluorescence spectra measured for different storage or fixation periods show a differential among those samples that were stored in water or were fixed either in formalin or methanol. The spectroscopic results indicate that formalin was the least disruptive fixative, having only a weak effect on the two-photon fluorescence spectroscopy of muscle tissue, whereas methanol had a significant influence on one of the autofluorescence peaks. The two handling processes yielded similar spectral information, indicating no different effects between them.

© 2000 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.6930) Medical optics and biotechnology : Tissue
(170.7160) Medical optics and biotechnology : Ultrafast technology

History
Original Manuscript: March 29, 2000
Revised Manuscript: July 7, 2000
Published: December 1, 2000

Citation
Ming G. Xu, Elizabeth D. Williams, Erik W. Thompson, and Min Gu, "Effect of handling and fixation processes on fluorescence spectroscopy of mouse skeletal muscles under two-photon excitation," Appl. Opt. 39, 6312-6317 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-34-6312


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