OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 34 — Dec. 1, 2000
  • pp: 6325–6331

Point-spread functions of a polarizing microscope equipped with high-numerical-aperture lenses

Rudolf Oldenbourg and Peter Török  »View Author Affiliations


Applied Optics, Vol. 39, Issue 34, pp. 6325-6331 (2000)
http://dx.doi.org/10.1364/AO.39.006325


View Full Text Article

Enhanced HTML    Acrobat PDF (11469 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

In an effort to establish the imaging properties of a new type of polarized-light microscope, we recorded images of small, uniaxial, birefringent crystals. We show that the sequence of in-focus and out-of-focus images, the so-called point-spread function, of a submicroscopic crystal can be used to measure the orientation of its optic axis in three-dimensional space. By analogy to conoscopic images out-of-focus images reveal the changes in relative phase shift between the extraordinary and the ordinary rays that propagate at different directions through the crystal. We also present simulated images of a pointlike anisotropic scattering particle and compare these with our experimental findings. The theoretical model is based on a complete vectorial theory for partial coherent imaging by use of polarized light and high-numerical-aperture lenses.

© 2000 Optical Society of America

OCIS Codes
(050.1940) Diffraction and gratings : Diffraction
(120.2130) Instrumentation, measurement, and metrology : Ellipsometry and polarimetry
(180.3170) Microscopy : Interference microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(260.1440) Physical optics : Birefringence

History
Original Manuscript: May 4, 2000
Revised Manuscript: August 16, 2000
Published: December 1, 2000

Citation
Rudolf Oldenbourg and Peter Török, "Point-spread functions of a polarizing microscope equipped with high-numerical-aperture lenses," Appl. Opt. 39, 6325-6331 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-34-6325


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. G. Mei, R. Oldenbourg, “Fast imaging polarimetry with precision universal compensator,” in Polarization Analysis and Measurement II, D. H. Goldstein, D. B. Chenault, eds., Proc. SPIE2265, 29–39 (1994). [CrossRef]
  2. Y. Otani, T. Shimada, T. Yoshizawa, N. Umeda, “Two-dimensional birefringence measurement using the phase shifting technique,” Opt. Eng. 33, 1604–1609 (1994). [CrossRef]
  3. M. Shribak, Y. Otani, T. Yoshizawa, “Return-path polarimeter for two dimensional birefringence distribution measurement,” in Polarization: Measurement, Analysis, and Remote Sensing II, Proc. SPIE3754, 144–149 (1999).
  4. B. Wang, T. C. Oakberg, “A new instrument for measuring both the magnitude and angle of low level linear birefringence,” Rev. Sci. Instrum. 70, 3847–3854 (1999). [CrossRef]
  5. G. Yao, L. V. Wang, “Two-dimensional depth-resolved Mueller matrix characterization of biological tissue by optical coherence tomography,” Opt. Lett. 24, 537–539 (1999). [CrossRef]
  6. D. A. Agard, Y. Hiraoka, P. Shaw, J. W. Sedat, “Fluorescence microscopy in three dimensions,” in Methods of Cell Biology, D. L. Taylor, Y.-L. Wang, eds. (Academic, San Diego, 1989), Vol. 30, pp. 353–377. [CrossRef]
  7. S. F. Gibson, F. Lanni, “Experimental test of an analytical model of aberration in an oil-immersion objective lens used in three-dimensional light microscopy,” J. Opt. Soc. Am. A 9, 154–166 (1992). [CrossRef] [PubMed]
  8. L. Tao, C. Nicholson, “The three-dimensional point spread functions of a microscope objective in image and object space,” J. Microsc. 178, 267–271 (1995). [CrossRef] [PubMed]
  9. P. Török, S. J. Hewlett, P. Varga, “The role of specimen-induced spherical aberration in confocal microscopy,” J. Microsc. 188, 158–172 (1997). [CrossRef]
  10. R. Oldenbourg, G. Mei, “New polarized light microscope with precision universal compensator,” J. Microsc. 180, 140–147 (1995). [CrossRef] [PubMed]
  11. P. Török, “Imaging of small birefringent objects by polarised light conventional and confocal microscopes,” Opt. Commun. 181, 7–18 (2000). [CrossRef]
  12. E. Hecht, Optics, 3rd ed. (Addison-Wesley, Reading, Mass., 1998).
  13. R. Oldenbourg, “A new view on polarization microscopy,” Nature 381, 811–812 (1996). [CrossRef] [PubMed]
  14. R. Oldenbourg, E. D. Salmon, P. T. Tran, “Birefringence of single and bundled microtubules,” Biophys. J. 74, 645–654 (1998). [CrossRef] [PubMed]
  15. S. Inoué, R. Oldenbourg, “Microscopes,” in Handbook of Optics, M. Bass, ed. (McGraw-Hill, New York, 1995), Vol. 2, pp. 17.1–17.52.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited