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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 34 — Dec. 1, 2000
  • pp: 6366–6368

Confocal imaging through weakly aberrating media

C. J. R. Sheppard  »View Author Affiliations


Applied Optics, Vol. 39, Issue 34, pp. 6366-6368 (2000)
http://dx.doi.org/10.1364/AO.39.006366


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Abstract

The effect on confocal imaging of spherical aberration caused by a weak refractive-index mismatch is discussed. Aberration balancing that uses a change in the objective’s tube length is studied. It is found that the range of depths that can be imaged satisfactorily by a high-numerical-aperture objective with compensation is an order of magnitude greater than that without compensation. The aberration balancing tends to break down for extremely high numerical apertures.

© 2000 Optical Society of America

OCIS Codes
(080.1010) Geometric optics : Aberrations (global)
(110.0180) Imaging systems : Microscopy
(110.2990) Imaging systems : Image formation theory
(180.1790) Microscopy : Confocal microscopy
(260.1960) Physical optics : Diffraction theory

History
Original Manuscript: April 3, 2000
Revised Manuscript: July 12, 2000
Published: December 1, 2000

Citation
C. J. R. Sheppard, "Confocal imaging through weakly aberrating media," Appl. Opt. 39, 6366-6368 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-34-6366


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References

  1. C. J. R. Sheppard, C. J. Cogswell, “Three-dimensional image formation in confocal microscopy,” J. Microsc. 159, 179–194 (1990). [CrossRef]
  2. C. J. R. Sheppard, M. Gu, “Aberration compensation in confocal microscopy,” Appl. Opt. 30, 3563–3568 (1991). [CrossRef] [PubMed]
  3. C. J. R. Sheppard, M. Gu, K. Brain, H. Zhou, “Influence of spherical aberration on axial imaging of confocal microscopy,” Appl. Opt. 33, 616–624 (1994). [CrossRef] [PubMed]
  4. P. Török, S. J. Hewlett, P. Varga, “The role of specimen-induced spherical aberration in confocal microscopy,” J. Microsc. 188, 158–172 (1997). [CrossRef]
  5. C. J. R. Sheppard, T. Wilson, “Effects of high angles of convergence on V(z) in the scanning acoustic microscope,” Appl. Phys. Lett. 38, 858–859 (1981). [CrossRef]
  6. M. Born, E. Wolf, Principles of Optics, 6th ed. (Pergamon, Oxford, 1993).

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