The effect on confocal imaging of spherical aberration caused by a weak refractive-index mismatch is discussed. Aberration balancing that uses a change in the objective’s tube length is studied. It is found that the range of depths that can be imaged satisfactorily by a high-numerical-aperture objective with compensation is an order of magnitude greater than that without compensation. The aberration balancing tends to break down for extremely high numerical apertures.
© 2000 Optical Society of America
(080.1010) Geometric optics : Aberrations (global)
(110.0180) Imaging systems : Microscopy
(110.2990) Imaging systems : Image formation theory
(180.1790) Microscopy : Confocal microscopy
(260.1960) Physical optics : Diffraction theory
C. J. R. Sheppard, "Confocal imaging through weakly aberrating media," Appl. Opt. 39, 6366-6368 (2000)