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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 34 — Dec. 1, 2000
  • pp: 6487–6497

Quantifying the absorption and reduced scattering coefficients of tissuelike turbid media over a broad spectral range with noncontact Fourier-transform hyperspectral imaging

Tuan H. Pham, Frederic Bevilacqua, Thorsten Spott, Jan S. Dam, Bruce J. Tromberg, and Stefan Andersson-Engels  »View Author Affiliations


Applied Optics, Vol. 39, Issue 34, pp. 6487-6497 (2000)
http://dx.doi.org/10.1364/AO.39.006487


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Abstract

Absorption (μ a ) and reduced scattering (μs) spectra of turbid media were quantified with a noncontact imaging approach based on a Fourier-transform interferometric imaging system (FTIIS). The FTIIS was used to collect hyperspectral images of the steady-state diffuse reflectance from turbid media. Spatially resolved reflectance data from Monte Carlo simulations were fitted to the recorded hyperspectral images to quantify μ a and μs spectra in the 550–850-nm region. A simple and effective calibration approach was introduced to account for the instrument response. With reflectance data that were close to and far from the source (0.5–6.5 mm), μ a and μs of homogeneous, semi-infinite turbid phantoms with optical property ranges comparable with those of tissues were determined with an accuracy of ±7% and ±3%, respectively. Prediction accuracy for μ a and μs degraded to ±12% and ±4%, respectively, when only reflectance data close to the source (0.5–2.5 mm) were used. Results indicate that reflectance data close to and far from the source are necessary for optimal quantification of μ a and μs. The spectral properties of μ a and μs values were used to determine the concentrations of absorbers and scatterers, respectively. Absorber and scatterer concentrations of two-chromophore turbid media were determined with an accuracy of ±5% and ±3%, respectively.

© 2000 Optical Society of America

OCIS Codes
(070.2590) Fourier optics and signal processing : ABCD transforms
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.0110) Medical optics and biotechnology : Imaging systems
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.7050) Medical optics and biotechnology : Turbid media

History
Original Manuscript: February 22, 2000
Revised Manuscript: September 7, 2000
Published: December 1, 2000

Citation
Tuan H. Pham, Frederic Bevilacqua, Thorsten Spott, Jan S. Dam, Bruce J. Tromberg, and Stefan Andersson-Engels, "Quantifying the absorption and reduced scattering coefficients of tissuelike turbid media over a broad spectral range with noncontact Fourier-transform hyperspectral imaging," Appl. Opt. 39, 6487-6497 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-34-6487

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