OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 36 — Dec. 20, 2000
  • pp: 6725–6737

Determination of the fluorescence quantum yield by oceanic phytoplankton in their natural habitat

Stéphane Maritorena, André Morel, and Bernard Gentili  »View Author Affiliations


Applied Optics, Vol. 39, Issue 36, pp. 6725-6737 (2000)
http://dx.doi.org/10.1364/AO.39.006725


View Full Text Article

Enhanced HTML    Acrobat PDF (205 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Sun-stimulated chlorophyll a fluorescence has been measured in situ, within the upward and downward light fields, in oceanic waters with chlorophyll concentrations of 0.04–3 mg m-3. We combined these signals with phytoplankton absorption spectra to derive the fluorescence quantum yield, ϕ (number of photons emitted by fluorescence/number of absorbed photons). ϕ was derived separately from hyperspectral (upward and downward) irradiance measurements (with a LI-COR Instruments spectroradiometer) and from nadir radiance near 683 nm (with a Biospherical Instruments profiler). The contribution of inelastic Raman scattering to the signal in the red band was assessed and subtracted. Raman-corrected ϕ values derived from the two instruments compared well. Vertical ϕ profiles were strongly structured, with maximal (5–6%) values at depth, whereas ϕ was ≅1% in near-surface waters (measurements made approximately at solar noon). These near-surface values are needed for interpretation of remotely sensed fluorescence signals. This optical study shows that the fluorescence yield of algae in their natural environment can be accurately derived in a nonintrusive way with available instrumentation and adequate protocols.

© 2000 Optical Society of America

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(260.2510) Physical optics : Fluorescence

History
Original Manuscript: March 16, 2000
Revised Manuscript: September 20, 2000
Published: December 20, 2000

Citation
Stéphane Maritorena, André Morel, and Bernard Gentili, "Determination of the fluorescence quantum yield by oceanic phytoplankton in their natural habitat," Appl. Opt. 39, 6725-6737 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-36-6725


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. J. E. Tyler, R. C. Smith, Measurements of Spectral Irradiance under Water, Vol. 1 of Ocean Series (Gordon & Breach, New York, 1970), p. 103.
  2. R. A. Neville, J. F. R. Gower, “Passive remote-sensing of phytoplankton via chlorophyll-alpha fluorescence,” J. Geophys. Res. 82, 3487–3493 (1977). [CrossRef]
  3. A. Morel, L. Prieur, “Analysis of variations in ocean color,” Limnol. Oceanogr. 22, 709–722 (1977). [CrossRef]
  4. H. R. Gordon, “Diffuse reflectance of the ocean: the theory of its augmentation by chlorophyll a fluorescence at 685 nm,” Appl. Opt. 18, 1161–1166 (1979). [CrossRef] [PubMed]
  5. G. W. Kattawar, J. C. Vastano, “Exact 1-D solution to the problem of chlorophyll fluorescence from the ocean,” Appl. Opt. 21, 2489–2492 (1982). [CrossRef] [PubMed]
  6. M. Kishino, S. Sugihara, N. Okami, “Influence of fluorescence of chlorophyll a on underwater upward irradiance spectrum,” La Mer 22, 224–232 (1984).
  7. M. Kishino, S. Sugihara, N. Okami, “Estimation of quantum yield of chlorophyll a fluorescence from the upward irradiance spectrum in the sea,” La Mer 22, 233–240 (1984).
  8. D. A. Kiefer, W. S. Chamberlin, C. R. Booth, “Natural fluorescence of chlorophyll a: relationship to photosynthesis and chlorophyll concentration in the western South Pacific gyre,” Limnol. Oceanogr. 34, 868–881 (1989). [CrossRef]
  9. P. G. Falkowski, D. A. Kiefer, “Chlorophyll a fluorescence in phytoplankton: relationship to photosynthesis and biomass,” J. Plankton Res. 7, 715–731 (1985). [CrossRef]
  10. W. S. Chamberlin, C. R. Booth, D. A. Kiefer, J. H. Morrow, R. C. Murphy, “Evidence for a simple relationship between natural fluorescence, photosynthesis and chlorophyll in the sea,” Deep-Sea Res. 37, 951–973 (1990). [CrossRef]
  11. B. J. Topliss, T. Platt, “Passive fluorescence and photosynthesis in the ocean—implications for remote-sensing,” Deep-Sea Res. 33, 849–864 (1986). [CrossRef]
  12. T. G. Owens, “Energy transformation and fluorescence in photosynthesis,” in Particle Analysis in Oceanography, S. Demers, ed. (Springer-Verlag, Berlin, 1991), pp. 101–137. [CrossRef]
  13. G. H. Krause, E. Weis, “Chlorophyll fluorescence and photosynthesis—the basics,” Annu. Rev. Plant Phys. 42, 313–349 (1991).
  14. J. F. R. Gower, G. A. Borstad, “Use of in vivo fluorescence line at 685 nm for remote sensing surveys of surface chlorophyll a,” in Oceanography from Space, J. F. R. Gower, ed. (Plenum, New York, 1981), pp. 329–338.
  15. M. R. Abbott, R. M. Letelier, “Chlorophyll fluorescence (MODIS Product Number 20),” MODIS algorithm theoretical basis document ( http://modis.gsfc.nasa.gov/MODIS/ATBD , 1999).
  16. M. Babin, A. Morel, B. Gentili, “Remote sensing of sea surface Sun-induced chlorophyll fluorescence: consequences of natural variations in the optical characteristics of phytoplankton and the quantum yield of chlorophyll a fluorescence,” Int. J. Remote Sens. 17, 2417–2448 (1996). [CrossRef]
  17. A. Bricaud, M. Babin, A. Morel, H. Claustre, “Variability in the chlorophyll-specific absorption coefficients of natural phytoplankton: analysis and parameterization,” J. Geophys. Res. 100, 13,321–13,332 (1995). [CrossRef]
  18. A. Bricaud, A. Morel, M. Babin, K. Allali, H. Claustre, “Variations of light absorption by suspended particles with chlorophyll a concentration in oceanic (case 1) waters: analysis and implications for bio-optical models,” J. Geophys. Res. 103, 31,033–31,044 (1998). [CrossRef]
  19. M. Kishino, M. Takahashi, N. Okami, S. Ichimura, “Estimation of the spectral absorption coefficients of phytoplankton in the sea,” Bull. Mar. Sci. 37, 634–642 (1985).
  20. A. Bricaud, D. Stramski, “Spectral absorption-coefficients of living phytoplankton and nonalgal biogenous matter—a comparison between the Peru upwelling area and the Sargasso Sea,” Limnol. Oceanogr. 35, 562–582 (1990). [CrossRef]
  21. H. Claustre, A. Morel, M. Babin, C. Cailliau, D. Marie, J.-C. Marty, D. Tailliez, D. Vaulot, “Variability in particle attenuation and chlorophyll fluorescence in the tropical Pacific: Scales, patterns, and biogeochemical implications,” J. Geophys. Res. 104, 3401–3422 (1999). [CrossRef]
  22. Y. Dandonneau, “Introduction to special section: biogeochemical conditions in the equatorial Pacific in late 1994,” J. Geophys. Res. 104, 3291–3295 (1999). [CrossRef]
  23. B. Coste, H. J. Minas, M. C. Bonin, “Production Pélagique des Côtes du Pérou et des Iles Galapagos, Campagne PACIPROD (8 Aout–18 Septembre 1986),” (Institut Français de Recherche pour l’Exploitation de la Mer, Service Documentation and Publications, Plouzane, France, 1987), p. 183.
  24. A. Morel, “Optical modeling of the upper ocean in relation to its biogenous matter content (Case I waters),” J. Geophys. Res. 93, 10,749–10,768 (1988). [CrossRef]
  25. D. J. Collins, D. A. Kiefer, J. B. SooHoo, I. S. McDermid, “The role of reabsorption in the spectral distribution of phytoplankton fluorescence emission,” Deep-Sea Res. 32, 983–1003 (1985). [CrossRef]
  26. R. M. Pope, E. S. Fry, “Absorption spectrum (380–700 nm) of pure water. II. Integrating cavity measurements,” Appl. Opt. 36, 8710–8723 (1997). [CrossRef]
  27. J. T. O. Kirk, “The upwelling light stream in natural waters,” Limnol. Oceanogr. 34, 1410–1425 (1989). [CrossRef]
  28. S. Maritorena, A. Morel, B. Gentili, “Diffuse reflectance of oceanic shallow waters: influence of water depth and bottom albedo,” Limnol. Oceanogr. 39, 1689–1703 (1994). [CrossRef]
  29. B. R. Marshall, R. C. Smith, “Raman scattering and in-water ocean optical properties,” Appl. Opt. 29, 71–84 (1990). [CrossRef] [PubMed]
  30. A. Morel, H. Loisel, “Apparent optical properties of oceanic water: dependence on the molecular scattering contribution,” Appl. Opt. 37, 4765–4776 (1998). [CrossRef]
  31. K. Allali, A. Bricaud, H. Claustre, “Spatial variations in the chlorophyll-specific absorption coefficients of phytoplankton and photosynthetically active pigments in the equatorial Pacific,” J. Geophys. Res. 102, 12,413–12,423 (1997). [CrossRef]
  32. C. S. Yentsch, D. A. Phinney, “A bridge between ocean optics and microbial ecology,” Limnol Oceanogr. 34, 1694–1705 (1989). [CrossRef]
  33. J. Marra, “Analysis of diel variability in chlorophyll fluorescence,” J. Mar. Res. 55, 767–784 (1997). [CrossRef]
  34. P. G. Falkowski, J. A. Raven, Aquatic photosynthesis (Blackwell, Malden, Mass., 1997), p. 375.
  35. D. A. Kiefer, R. A. Reynolds, “Advances in understanding phytoplankton fluorescence and photosynthesis,” in Primary Productivity and Biogeochemical Cycles in the Sea, P. G. Falkowski, A. D. Woodhead, eds. (Plenum, New York, 1992), pp. 155–179. [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited