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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 40, Iss. 18 — Jun. 20, 2001
  • pp: 3005–3013

Fluorescence from airborne microparticles: dependence on size, concentration of fluorophores, and illumination intensity

Steven C. Hill, Ronald G. Pinnick, Stanley Niles, Nicholas F. Fell, Jr., Yong-Le Pan, Jerold Bottiger, Burt V. Bronk, Stephen Holler, and Richard K. Chang  »View Author Affiliations


Applied Optics, Vol. 40, Issue 18, pp. 3005-3013 (2001)
http://dx.doi.org/10.1364/AO.40.003005


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Abstract

We measured fluorescence from spherical water droplets containing tryptophan and from aggregates of bacterial cells and compared these measurements with calculations of fluorescence of dielectric spheres. The measured dependence of fluorescence on size, from both droplets and dry-particle aggregates of bacteria, is proportional to the absorption cross section calculated for homogeneous spheres containing the appropriate percentage of tryptophan. However, as the tryptophan concentration of the water droplets is increased, the measured fluorescence from droplets increases less than predicted, probably because of concentration quenching. We model the dependence of the fluorescence on input intensity by assuming that the average time between fluorescence emission events is the sum of the fluorescence lifetime and the excitation lifetime (the average time it takes for an illuminated molecule to be excited), which we calculated assuming that the intensity inside the particle is uniform. Even though the intensity inside the particles spatially varies, this assumption of uniform intensity still leads to results consistent with the measured intensity dependence.

© 2001 Optical Society of America

OCIS Codes
(010.1100) Atmospheric and oceanic optics : Aerosol detection
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(290.4020) Scattering : Mie theory
(290.5850) Scattering : Scattering, particles
(290.5860) Scattering : Scattering, Raman
(300.2530) Spectroscopy : Fluorescence, laser-induced

History
Original Manuscript: November 3, 2000
Revised Manuscript: February 28, 2001
Published: June 20, 2001

Citation
Steven C. Hill, Ronald G. Pinnick, Stanley Niles, Nicholas F. Fell, Yong-Le Pan, Jerold Bottiger, Burt V. Bronk, Stephen Holler, and Richard K. Chang, "Fluorescence from airborne microparticles: dependence on size, concentration of fluorophores, and illumination intensity," Appl. Opt. 40, 3005-3013 (2001)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-40-18-3005

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