OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 40, Iss. 21 — Jul. 20, 2001
  • pp: 3586–3597

Spatial distribution of fluorescence intensity within large droplets and its dependence on dye concentration

Roland Domann and Yannis Hardalupas  »View Author Affiliations


Applied Optics, Vol. 40, Issue 21, pp. 3586-3597 (2001)
http://dx.doi.org/10.1364/AO.40.003586


View Full Text Article

Enhanced HTML    Acrobat PDF (1880 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The dependence of fluorescence intensity distributions within droplets on added dye concentration has been calculated by extension of the geometrical-optics approximation and verified by experimental observations. With rising dye concentration, surface plots of the equatorial fluorescence pattern show decreasing relevance of intensity enhancement at focusing points of internal light rays and increasing effects of linear absorption on the characteristic features of the distribution. For comparison with experimentally obtained images of the fluorescence intensity distribution within droplets, a method for calculating volume-integrated intensity distributions was developed in which image distortion at the fluid–air interface is included. A comparison of the calculated and the experimentally determined fluorescence intensity distributions within a droplet confirmed the accuracy of the geometrical-optics approach at high dye concentrations. However, discrepancies from experimental results are visible at low dye concentrations owing to nonlinear optical effects.

© 2001 Optical Society of America

OCIS Codes
(080.0080) Geometric optics : Geometric optics
(100.3020) Image processing : Image reconstruction-restoration
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(180.2520) Microscopy : Fluorescence microscopy
(290.0290) Scattering : Scattering
(350.4990) Other areas of optics : Particles

History
Original Manuscript: June 13, 2000
Revised Manuscript: March 9, 2001
Published: July 20, 2001

Citation
Roland Domann and Yannis Hardalupas, "Spatial distribution of fluorescence intensity within large droplets and its dependence on dye concentration," Appl. Opt. 40, 3586-3597 (2001)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-40-21-3586

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited