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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 40, Iss. 24 — Aug. 20, 2001
  • pp: 3969–3983

Fluorescence correlation spectroscopy: inception, biophysical experimentations, and prospectus

Watt W. Webb  »View Author Affiliations


Applied Optics, Vol. 40, Issue 24, pp. 3969-3983 (2001)
http://dx.doi.org/10.1364/AO.40.003969


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Abstract

Fluorescence correlation spectroscopy examines the chemical and the photophysical dynamics of dilute molecular solutions by measurement of the dynamic optical fluctuations of the fluorescence of a few molecules, even averaging less than one molecule at a time, in open focal volumes that are usually less than a femtoliter (<10-18 m3). It applies the same principles of statistical thermodynamics as does quasi-elastic light scattering. Molecular interactions, conformational changes, chemical reactions, and photophysical dynamics that are not ordinarily detectable by quasi-elastic light scattering can be analyzed by fluorescence correlation spectroscopy in cases in which molecular fluorescence changes in the dynamic range 10-7–102 s.

© 2001 Optical Society of America

OCIS Codes
(030.0030) Coherence and statistical optics : Coherence and statistical optics
(170.1580) Medical optics and biotechnology : Chemometrics
(180.0180) Microscopy : Microscopy
(270.2500) Quantum optics : Fluctuations, relaxations, and noise
(300.0300) Spectroscopy : Spectroscopy

History
Original Manuscript: January 31, 2001
Revised Manuscript: April 26, 2001
Published: August 20, 2001

Citation
Watt W. Webb, "Fluorescence correlation spectroscopy: inception, biophysical experimentations, and prospectus," Appl. Opt. 40, 3969-3983 (2001)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-40-24-3969

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