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Fluorescence correlation spectroscopy: inception, biophysical experimentations, and prospectus

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Abstract

Fluorescence correlation spectroscopy examines the chemical and the photophysical dynamics of dilute molecular solutions by measurement of the dynamic optical fluctuations of the fluorescence of a few molecules, even averaging less than one molecule at a time, in open focal volumes that are usually less than a femtoliter (<10-18 m3). It applies the same principles of statistical thermodynamics as does quasi-elastic light scattering. Molecular interactions, conformational changes, chemical reactions, and photophysical dynamics that are not ordinarily detectable by quasi-elastic light scattering can be analyzed by fluorescence correlation spectroscopy in cases in which molecular fluorescence changes in the dynamic range 10-7–102 s.

© 2001 Optical Society of America

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