Fiber-optic, polarized elastic-scattering spectroscopy techniques are implemented and demonstrated as a method for determining both scatterer size and concentration in highly scattering media. Measurements of polystyrene spheres are presented to validate the technique. Measurements of biological cells provide an estimate of the average effective scatterer radius of 0.5–1.0 μm. This average effective scatterer size is significantly smaller than the nucleus. In addition, to facilitate use of polarization techniques on biological cells, polarized angular dependent scattering from cell suspensions was measured. The light scattering from cells has properties similar to those of small spheres.
© 2001 Optical Society of America
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.7050) Medical optics and biotechnology : Turbid media
Judith R. Mourant, Tamara M. Johnson, and James P. Freyer, "Characterizing Mammalian Cells and Cell Phantoms by Polarized Backscattering Fiber-Optic Measurements," Appl. Opt. 40, 5114-5123 (2001)