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Applied Optics

Applied Optics


  • Vol. 40, Iss. 31 — Nov. 1, 2001
  • pp: 5650–5657

Objective-type dark-field illumination for scattering from microbeads

Ido Braslavsky, Roee Amit, B. M. Jaffar Ali, Opher Gileadi, Amos Oppenheim, and Joel Stavans  »View Author Affiliations

Applied Optics, Vol. 40, Issue 31, pp. 5650-5657 (2001)

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We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a signal-to-noise ratio of 6 and detected 20-nm gold particles with a signal-to-noise ratio of 5. We illustrate the method’s use by following the Brownian motion of small beads attached by short DNA tethers to a substrate.

© 2001 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(120.1880) Instrumentation, measurement, and metrology : Detection
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(290.5850) Scattering : Scattering, particles

Original Manuscript: January 22, 2001
Revised Manuscript: July 6, 2001
Published: November 1, 2001

Ido Braslavsky, Roee Amit, B. M. Jaffar Ali, Opher Gileadi, Amos Oppenheim, and Joel Stavans, "Objective-type dark-field illumination for scattering from microbeads," Appl. Opt. 40, 5650-5657 (2001)

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