Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Noninvasive measurement of fluorophore concentration in turbid media with a simple fluorescence/reflectance ratio technique

Not Accessible

Your library or personal account may give you access

Abstract

Measurement of the concentration of fluorescent compounds in turbid media is difficult because the absorption and multiple scattering of excitation and emission of light has a large effect on the detected fluorescence. For surface measurements with optical fibers we demonstrate by experiments and numerical simulation that this effect can be minimized by measurement of the fluorescence at one source–detector distance, the diffusely reflected excitation light at a second distance, and with the ratio of these two signals as an indicator of fluorophore concentration. For optical properties typical of soft tissue in the red and the near infrared the optimum performance is obtained by measurement of fluorescence at 0.65 mm and reflectance at 1.35 mm. This choice reduces the rms error in fluorophore concentration to 14.6% over a wide range of absorption and scattering coefficients.

© 2001 Optical Society of America

Full Article  |  PDF Article
More Like This
Quantification of fluorophore concentration in tissue-simulating media by fluorescence measurements with a single optical fiber

Kevin R. Diamond, Michael S. Patterson, and Thomas J. Farrell
Appl. Opt. 42(13) 2436-2442 (2003)

Intrinsic fluorescence spectroscopy in turbid media: disentangling effects of scattering and absorption

Markus G. Müller, Irene Georgakoudi, Qingguo Zhang, Jun Wu, and Michael S. Feld
Appl. Opt. 40(25) 4633-4646 (2001)

Fluorescence spectroscopy of tissue: recovery of intrinsic fluorescence from measured fluorescence

Craig M. Gardner, Steven L. Jacques, and Ashley J. Welch
Appl. Opt. 35(10) 1780-1792 (1996)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved