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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 40, Iss. 34 — Dec. 1, 2001
  • pp: 6396–6405

Absorption coefficient and purine photobleaching rate in colon mucosa during resonance Raman spectroscopy at 251 nm

Nada N. Boustany  »View Author Affiliations


Applied Optics, Vol. 40, Issue 34, pp. 6396-6405 (2001)
http://dx.doi.org/10.1364/AO.40.006396


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Abstract

In contrast to spectroscopy at longer wavelengths, typical attributes of ultraviolet resonance Raman (UVRR) spectroscopy of biologic tissue are higher absorption coefficient, μ, and higher photobleaching rate, κ. This study was aimed at measuring μ and κ during UVRR spectroscopy of human colon tissue at 251 nm. μ was used to estimate the penetration depth of the excitation light; κ was used to predict the rate of signal decrease that was due to photobleaching as a function of laser fluence and tissue thickness. The fitting of the equations through description of a three-state transition model to experimental data that consisted of a purine UVRR signal gave μ = 0.0169 ± 0.0023 µm-1 and κ = 0.572 ± 0.168 (mJ/µm2)-1. κ remained independent of power P for P < 1 mW, but higher power values resulted in a higher photobleaching rate. As predicted by the model, signal decrease that was due to photobleaching was slower as sample thickness was increased.

© 2001 Optical Society of America

OCIS Codes
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(260.5130) Physical optics : Photochemistry
(260.5740) Physical optics : Resonance
(260.7190) Physical optics : Ultraviolet
(300.6450) Spectroscopy : Spectroscopy, Raman

History
Original Manuscript: May 1, 2001
Revised Manuscript: July 23, 2001
Published: December 1, 2001

Citation
Nada N. Boustany, "Absorption coefficient and purine photobleaching rate in colon mucosa during resonance Raman spectroscopy at 251 nm," Appl. Opt. 40, 6396-6405 (2001)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-40-34-6396


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