Time-resolved reflectance spectroscopy can be used to assess nondestructively the bulk (rather than the superficial) optical properties of highly diffusive media. A fully automated system for time-resolved reflectance spectroscopy was used to evaluate the absorption and the transport scattering spectra of fruits in the red and the near-infrared regions. In particular, data were collected in the range 650–1000 nm from three varieties of apples and from peaches, kiwifruits, and tomatoes. The absorption spectra were usually dominated by the water peak near 970 nm, whereas chlorophyll was detected at 675 nm. For all species the scattering decreased progressively with increasing wavelength. A best fit to water and chlorophyll absorption line shapes and to Mie theory permitted the estimation of water and chlorophyll content and the average size of scattering centers in the bulk of intact fruits.
© 2001 Optical Society of America
(170.5280) Medical optics and biotechnology : Photon migration
(170.7050) Medical optics and biotechnology : Turbid media
(290.0290) Scattering : Scattering
(300.1030) Spectroscopy : Absorption
(300.6500) Spectroscopy : Spectroscopy, time-resolved
Rinaldo Cubeddu, Cosimo D’Andrea, Antonio Pifferi, Paola Taroni, Alessandro Torricelli, Gianluca Valentini, Colin Dover, David Johnson, Margarita Ruiz-Altisent, and Constantino Valero, "Nondestructive Quantification of Chemical and Physical Properties of Fruits by Time-Resolved Reflectance Spectroscopy in the Wavelength Range 650–1000 nm," Appl. Opt. 40, 538-543 (2001)