OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 40, Iss. 4 — Feb. 1, 2001
  • pp: 570–582

Resonant-enhanced evanescent-wave fluorescence biosensing with cylindrical optical cavities

Steve Blair and Yan Chen  »View Author Affiliations


Applied Optics, Vol. 40, Issue 4, pp. 570-582 (2001)
http://dx.doi.org/10.1364/AO.40.000570


View Full Text Article

Enhanced HTML    Acrobat PDF (603 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We show that the artificial resonances of dielectric optical cavities can be used to enhance the detection sensitivity of evanescent-wave optical fluorescence biosensors to the binding of a labeled analyte with a biospecific monolayer. Resonant coupling of power into the optical cavity allows for efficient use of the long photon lifetimes (or equivalently, the high internal power) of the high-Q whispering gallery modes to increase the probability of photon absorption into the fluorophore, thereby enhancing fluorescence emission. A method to compare the intrinsic sensitivity between resonant cavity and waveguide formats is also developed. Using realistic estimates for dielectric cylindrical cavities in both bulk and integrated configurations, we can expect sensitivity enhancement by at least an order of magnitude over standard waveguide evanescent sensors of equivalent sensing geometries. In addition, the required sample volume can be reduced significantly. The cylindrical cavity format is compatible with a large variety of sensing modalities such as immunoassay and molecular diagnostic assay.

© 2001 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(230.5750) Optical devices : Resonators

History
Original Manuscript: March 29, 2000
Revised Manuscript: June 26, 2000
Published: February 1, 2001

Citation
Steve Blair and Yan Chen, "Resonant-enhanced evanescent-wave fluorescence biosensing with cylindrical optical cavities," Appl. Opt. 40, 570-582 (2001)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-40-4-570

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited