Simultaneous measurement of one- and two-photon excited fluorescence from a single sample: a detection method for oligonucleotides
Applied Optics, Vol. 41, Issue 12, pp. 2285-2291 (2002)
http://dx.doi.org/10.1364/AO.41.002285
Acrobat PDF (270 KB)
Abstract
A new method has been developed that is based on the use of a single-excitation wavelength from a cw laser to excite simultaneously one-photon and two-photon fluorescence (TPF). Fluorescence bands of a sample containing two oligonucleotides, one labeled with a one-photon fluorescence dye and the other with a TPF dye, can be measured simultaneously. The two fluorescence bands are well separated, because the one-photon excited fluorescence band is redshifted, whereas the TPF band is blueshifted from the excitation wavelength. The spectral separation was found to be as large as 200 nm when ADS 840NCS was used to label one oligonucleotide for one-photon fluorescence and Rhodamine Red-X dye was used for TPF. Spectral overlapping problem that plagues current DNA sequencing techniques can be eliminated effectively with this method.
© 2002 Optical Society of America
OCIS Codes
(000.1570) General : Chemistry
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(140.0140) Lasers and laser optics : Lasers and laser optics
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(230.1040) Optical devices : Acousto-optical devices
(300.0300) Spectroscopy : Spectroscopy
Citation
Troy Alexander and Chieu D. Tran, "Simultaneous measurement of one- and two-photon excited fluorescence from a single sample: a detection method for oligonucleotides," Appl. Opt. 41, 2285-2291 (2002)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-41-12-2285
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Ultrahigh long-term dimensional stability of a sapphire cryogenic optical resonator (OL)
- Quantum-Cascade Laser Measurements of Stratospheric Methane and Nitrous Oxide (AO)
- Laser Applications to Chemical, Security, and Environmental Analysis: introduction to the feature issue (AO)
- Laser Applications to Chemical, Security, and Environmental Analysis: introduction to the feature issue (AO)
- Laser Applications to Chemical, Security, and Environmental Analysis: introduction to the feature issue (AO)
Related Conference Papers
- Multiphonon Relaxation Studies of 4I11/2 and 4I13/2 Energy Levels in Er,RE:YAG Laser Crystals
- Diode Laser Stabilization for Coherent Driving of Rare Earth Ions
- Mid-IR High-Resolution Intracavity Cr2+:ZnSe Laser-Based Spectrometer
- Widely Tunable Cr2+:ZnSe Laser Source for Trace-Gas Sensing
- Precisely-Controlled Frequency Agile Laser for Radio Frequency Spectral Analysis
« Previous Article | Next Article »
OSA is a member of 