We report on a quantitative comparison between the single-source and the dual-interfering-source configurations for the detection of fluorescent heterogeneities embedded in a piecewise highly scattering homogeneous fluorescent background. The study is based on simulations with analytical solutions of the frequency-domain fluorescent diffuse photon density waves and practical signal-to-noise ratio considerations. Results show that dual-interfering sources outperform single-source techniques for the detection of heterogeneities in terms of fluorophore concentration and lifetime contrast. To detect the same inhomogeneity, less concentration and lifetime contrast is required with dual-interfering sources.
© 2002 Optical Society of America
(110.5100) Imaging systems : Phased-array imaging systems
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.5270) Medical optics and biotechnology : Photon density waves
(170.5280) Medical optics and biotechnology : Photon migration
(260.2510) Physical optics : Fluorescence
Xavier Intes, Yu Chen, Xingde Li, and Britton Chance, "Detection limit enhancement of fluorescent heterogeneities in turbid media by dual-interfering excitation," Appl. Opt. 41, 3999-4007 (2002)