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Applied Optics

Applied Optics


  • Vol. 41, Iss. 19 — Jul. 1, 2002
  • pp: 4024–4035

Determination of optical parameters of human breast tissue from spatially resolved fluorescence: a diffusion theory model

Maya S. Nair, Nirmalya Ghosh, Narisetti Sundar Raju, and Asima Pradhan  »View Author Affiliations

Applied Optics, Vol. 41, Issue 19, pp. 4024-4035 (2002)

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We report the measurement of optical transport parameters of pathologically characterized malignant tissues, normal tissues, and different types of benign tumors of the human breast in the visible wavelength region. A spatially resolved steady-state diffuse fluorescence reflectance technique was used to estimate the values for the reduced-scattering coefficient (μ s ′) and the absorption coefficient (μ a ) of human breast tissues at three wavelengths (530, 550, and 590 nm). Different breast tissues could be well differentiated from one another, and different benign tumors could also be distinguished by their measured transport parameters. A diffusion theory model was developed to describe fluorescence light energy distribution, especially its spatial variation in a turbid and multiply scattering medium such as human tissue. The validity of the model was checked with a Monte Carlo simulation and also with different tissue phantoms prepared with polystyrene microspheres as scatterers, riboflavin as fluorophores, and methylene blue as absorbers.

© 2002 Optical Society of America

OCIS Codes
(160.4760) Materials : Optical properties
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(260.2510) Physical optics : Fluorescence
(290.1990) Scattering : Diffusion

Original Manuscript: May 11, 2001
Revised Manuscript: November 9, 2001
Published: July 1, 2002

Maya S. Nair, Nirmalya Ghosh, Narisetti Sundar Raju, and Asima Pradhan, "Determination of optical parameters of human breast tissue from spatially resolved fluorescence: a diffusion theory model," Appl. Opt. 41, 4024-4035 (2002)

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