An optical system based on short-coherence digital holography suitable for three-dimensional (3D) microscopic investigations is described. The light source is a short-coherence laser, and the holograms are recorded on a CCD sensor. The interference (hologram) occurs only when the path lengths of the reference and the object beam are matched within the coherence length of the laser. The image of the part of the sample that matches the reference beam is reconstructed by numerical evaluation of the hologram. The advantages of the method are high numerical aperture (this means high spatial resolution), detection of the 3D shape, and a lensless imaging system. Experimental results are presented.
© 2002 Optical Society of America
(070.0070) Fourier optics and signal processing : Fourier optics and signal processing
(090.0090) Holography : Holography
(090.1760) Holography : Computer holography
(100.3010) Image processing : Image reconstruction techniques
(180.0180) Microscopy : Microscopy
Giancarlo Pedrini and Hans J. Tiziani, "Short-Coherence Digital Microscopy by Use of a Lensless Holographic Imaging System," Appl. Opt. 41, 4489-4496 (2002)