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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 41, Iss. 22 — Aug. 1, 2002
  • pp: 4704–4708

High-Speed 1-Frame/ms Scanning Confocal Microscope with a Microlens and Nipkow Disks

Takeo Tanaami, Shinya Otsuki, Nobuhiro Tomosada, Yasuhito Kosugi, Mizuho Shimizu, and Hideyuki Ishida  »View Author Affiliations


Applied Optics, Vol. 41, Issue 22, pp. 4704-4708 (2002)
http://dx.doi.org/10.1364/AO.41.004704


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Abstract

We have developed a high-speed confocal laser microscope. A microlens-array disk set in front of a pinhole-array disk improved optical efficiency more than ten times compared with that of conventional Nipkow confocal microscopy. This new microscope achieves a high-speed measurement of 1 frame/ms. We expect that it will be used for measuring biological and industrial active samples.

© 2002 Optical Society of America

OCIS Codes
(120.5800) Instrumentation, measurement, and metrology : Scanners
(170.0110) Medical optics and biotechnology : Imaging systems
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy

Citation
Takeo Tanaami, Shinya Otsuki, Nobuhiro Tomosada, Yasuhito Kosugi, Mizuho Shimizu, and Hideyuki Ishida, "High-Speed 1-Frame/ms Scanning Confocal Microscope with a Microlens and Nipkow Disks," Appl. Opt. 41, 4704-4708 (2002)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-41-22-4704


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References

  1. R. C. Mellors and R. Silver, “A microfluorometric scanner for the differential detection of cells,” Science 114, 356–361 (1951).
  2. T. Tanaami, Y. Sugiyama, and K. Mikuriya, “High-speed confocal laser microscopy,” Yokogawa Tech. Rep. 9, English ed. (Yokogawa Electric Corporation, Tokyo, Japan, 1994), pp. 7–10.
  3. A. Ichihara, T. Tanaami, K. Isozaki, Y. Sugiyama, Y. Kosugi, K. Mikuriya, M. Abe, and I. Umeda, “High-speed confocal fluorescent microscopy using a Nipkow scanner with microlenses for 3-d imaging of single fluorescent molecule in real time,” Bioimages 4(2), 57–62 (1996).
  4. C. Genka, H. Ishida, K. Ichimori, Y. Hirota, T. Tanaami, and H. Nakazawa, “Visualization of biphasic Ca2+ diffusion from cytesol to nucleus in contracting adult rat cardiac myocytes with an ultra-fast confocal imaging system,” Cell Calcium 25, 199–208 (1999).

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