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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 41, Iss. 22 — Aug. 1, 2002
  • pp: 4704–4708

High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks

Takeo Tanaami, Shinya Otsuki, Nobuhiro Tomosada, Yasuhito Kosugi, Mizuho Shimizu, and Hideyuki Ishida  »View Author Affiliations


Applied Optics, Vol. 41, Issue 22, pp. 4704-4708 (2002)
http://dx.doi.org/10.1364/AO.41.004704


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Abstract

We have developed a high-speed confocal laser microscope. A microlens-array disk set in front of a pinhole-array disk improved optical efficiency more than ten times compared with that of conventional Nipkow confocal microscopy. This new microscope achieves a high-speed measurement of 1 frame/ms. We expect that it will be used for measuring biological and industrial active samples.

© 2002 Optical Society of America

OCIS Codes
(120.5800) Instrumentation, measurement, and metrology : Scanners
(170.0110) Medical optics and biotechnology : Imaging systems
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy

History
Original Manuscript: November 16, 2001
Revised Manuscript: March 14, 2002
Published: August 1, 2002

Citation
Takeo Tanaami, Shinya Otsuki, Nobuhiro Tomosada, Yasuhito Kosugi, Mizuho Shimizu, and Hideyuki Ishida, "High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks," Appl. Opt. 41, 4704-4708 (2002)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-41-22-4704

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