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Applied Optics

Applied Optics


  • Vol. 41, Iss. 22 — Aug. 1, 2002
  • pp: 4712–4721

Multiple-fiber probe design for fluorescence spectroscopy in tissue

T. Joshua Pfefer, Kevin T. Schomacker, Marwood N. Ediger, and Norman S. Nishioka  »View Author Affiliations

Applied Optics, Vol. 41, Issue 22, pp. 4712-4721 (2002)

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The fiber-optic probe is an essential component of many quantitative fluorescence spectroscopy systems, enabling delivery of excitation light and collection of remitted fluorescence in a wide variety of clinical and laboratory situations. However, there is little information available on the role of illumination-collection geometry to guide the design of these components. Therefore we used a Monte Carlo model to investigate the effect of multifiber probe design parameters—numerical aperture, fiber diameter, source-collection fiber separation distance, and fiber-tissue spacer thickness—on light propagation and the origin of detected fluorescence. An excitation wavelength of 400 nm and an emission wavelength of 630 nm were simulated. Noteworthy effects included an increase in axial selectivity with decreasing fiber size and a transition with increasing fiber-tissue spacer size from a subsurface peak in fluorophore sensitivity to a nearly monotonic decrease typical of single-fiber probes. We provide theoretical evidence that probe design strongly affects tissue interrogation. Therefore application-specific customization of probe design may lead to improvements in the efficacy of fluorescence-based diagnostic devices.

© 2002 Optical Society of America

OCIS Codes
(000.3860) General : Mathematical methods in physics
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

Original Manuscript: February 5, 2002
Revised Manuscript: April 3, 2002
Published: August 1, 2002

T. Joshua Pfefer, Kevin T. Schomacker, Marwood N. Ediger, and Norman S. Nishioka, "Multiple-fiber probe design for fluorescence spectroscopy in tissue," Appl. Opt. 41, 4712-4721 (2002)

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