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Applied Optics

Applied Optics


  • Vol. 41, Iss. 31 — Nov. 1, 2002
  • pp: 6725–6730

Method for evaluating optical characteristics of endoscopes for recording fluorescence-related cardiac electrical activity

Soma Sau, Howard I. Bassen, and Victor Krauthamer  »View Author Affiliations

Applied Optics, Vol. 41, Issue 31, pp. 6725-6730 (2002)

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Nondestructive methods were used to evaluate marketed fiber-optic endoscopes (intended for simple viewing) for fluorescence recording. Our application is for optical recording from the heart. For one angioscope, we measured a focal length of 0.33 mm, a field of view of 45°, an aperture of 0.26 mm, and an efficiency of 43%. We calculated that the angioscope would give a signal-to-noise ratio of 1.0 for a cardiac action potential, if its field of view were divided into a nine-pixel array (for safe continuous illumination). Our methods are useful in designing and evaluating fluorescence fiber-optic systems with superior signal quality and spatial resolution.

© 2002 Optical Society of America

OCIS Codes
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.5380) Medical optics and biotechnology : Physiology
(260.2510) Physical optics : Fluorescence

Original Manuscript: January 10, 2002
Revised Manuscript: June 3, 2002
Published: November 1, 2002

Soma Sau, Howard I. Bassen, and Victor Krauthamer, "Method for evaluating optical characteristics of endoscopes for recording fluorescence-related cardiac electrical activity," Appl. Opt. 41, 6725-6730 (2002)

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  1. L. B. Cohen, S. Lesher, “Optical monitoring of membrane potential: methods of multisite optical measurement,” Soc. Gen. Physiol. Ser. 40, 71–99 (1986). [PubMed]
  2. B.-R. Choi, G. Salama, “Simultaneous maps of optical action potentials and calcium transients in guinea-pig hearts: mechanisms underlying concordant alterans,” J. Physiol. (London) 529, 171–188 (2000). [CrossRef]
  3. T. A. Bowmaster, C. C. Davis, V. Krauthamer, “Excitation and detection of action potential induced fluorescence changes through a single monomode optical fiber,” Biochim. Biophys. Acta 1091, 9–14 (1991). [CrossRef] [PubMed]
  4. V. Krauthamer, H. J. Bryant, C. C. Davis, T. W. Athey, “Action potential-induced fluorescence changes resolved with an optical fiber carrying excitation light,” J. Fluoresc. 1, 207–213 (1991). [CrossRef] [PubMed]
  5. V. Krauthamer, “Apparatus for fluorescent excitation and detection from potentiometric dyes with a single-ended optical fiber,” U.S. patent5,239,998 (31August1993).
  6. V. Krauthamer, J. L. Jones, “Calcium dynamics in cultured heart cells exposed to high-voltage electric shocks,” Life Sci. 60, 1977–1985 (1997). [CrossRef]
  7. V. Ntzachristos, B. Chance, “Probing physiology and molecular function using optical imaging: applications to breast cancer,” Breast Can. Res. 3, 41–46 (2001). [CrossRef]
  8. C. A. Combs, R. S. Balaban, “Direct imaging of dehydrogenase activity within living cells using enzyme-dependent fluorescence recovery after photobleaching,” Biophys. J. 80, 2018–2028 (2001). [CrossRef] [PubMed]
  9. H. Bassen, V. Krauthamer, “Apparatus and method of in situ detection of areas of cardiac electrical activity,” U.S. patent5,678,550 (21October1997).
  10. E. Fluhler, V. G. Burnaham, L. M. Loew, “Spectra, membrane binding, and potentiometric responses of new charge shift probes,” Biochemistry 24, 5749–5755 (1985). [CrossRef] [PubMed]
  11. H. H. Hopkins, “Physics of the fiberoptic endoscope,” in Endoscopy, G. Berci, ed. (Appleton-Century-Croft, New York, 1976), pp. 27–63.
  12. V. Krauthamer, C. C. Davis, E.-T. Gan, “Two-point electrical-fluorescence recording from myocardium with optical fibers,” IEEE Trans. Biomed. Eng. 41, 1191–1193 (1994). [CrossRef] [PubMed]

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