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Applied Optics

Applied Optics


  • Vol. 41, Iss. 34 — Dec. 2, 2002
  • pp: 7334–7338

Determination of bacterial activity by use of an evanescent-wave fiber-optic sensor

M. Shelly John, Anil Kishen, Lim Chu Sing, and Anand Asundi  »View Author Affiliations

Applied Optics, Vol. 41, Issue 34, pp. 7334-7338 (2002)

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A novel technique based on fiber-optic evanescent-wave spectroscopy is proposed for the detection of bacterial activity in human saliva. The sensor determines the specific concentration of Streptococcus mutans in saliva, which is a major causative factor in dental caries. In this design, one prepares the fiber-optic bacterial sensor by replacing a portion of the cladding region of a multimode fiber with a dye-encapsulated xerogel, using the solgel technique. The exponential decay of the evanescent wave at the core-cladding interface of a multimode fiber is utilized for the determination of bacterial activity in saliva. The acidogenic profile of Streptococcus mutans is estimated by use of evanescent-wave absorption spectra at various levels of bacterial activity.

© 2002 Optical Society of America

OCIS Codes
(060.2310) Fiber optics and optical communications : Fiber optics
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(170.1850) Medical optics and biotechnology : Dentistry
(300.1030) Spectroscopy : Absorption
(300.6550) Spectroscopy : Spectroscopy, visible

Original Manuscript: April 19, 2002
Revised Manuscript: July 29, 2002
Published: December 1, 2002

M. Shelly John, Anil Kishen, Lim Chu Sing, and Anand Asundi, "Determination of bacterial activity by use of an evanescent-wave fiber-optic sensor," Appl. Opt. 41, 7334-7338 (2002)

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