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Applied Optics

Applied Optics


  • Vol. 41, Iss. 4 — Feb. 1, 2002
  • pp: 805–812

High-resolution full-field optical coherence tomography with a Linnik microscope

Arnaud Dubois, Laurent Vabre, Albert-Claude Boccara, and Emmanuel Beaurepaire  »View Author Affiliations

Applied Optics, Vol. 41, Issue 4, pp. 805-812 (2002)

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We describe an original microscope for high-resolution optical coherence tomography applications. Our system is based on a Linnik interference microscope with high-numerical-aperture objectives. Lock-in detection of the interference signal is achieved in parallel on a CCD by use of a photoelastic birefringence modulator and full-field stroboscopic illumination with an infrared LED. Transverse cross-section (en-face, or XY) images can be obtained in real time with better than 1-µm axial (Z) resolution and 0.5-µm transverse (XY) resolution. A sensitivity of ∼80 dB is reached at a 1-image/s acquisition rate, which allows tomography in scattering media such as biological tissues.

© 2002 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.3170) Microscopy : Interference microscopy

Original Manuscript: June 4, 2001
Revised Manuscript: September 24, 2001
Published: February 1, 2002

Arnaud Dubois, Laurent Vabre, Albert-Claude Boccara, and Emmanuel Beaurepaire, "High-resolution full-field optical coherence tomography with a Linnik microscope," Appl. Opt. 41, 805-812 (2002)

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  1. T. Wilson, C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy (Academic, New York, 1984).
  2. T. Wilson, Confocal Microscopy (Academic, London, 1990).
  3. J. Pawley, ed., The Handbook of Confocal Microscopy, 2nd ed. (Plenum, New York, 1995), p. 445.
  4. K. Creath, “Phase-measurements interferometry techniques,” in Progress in Optics, E. Wolf, ed. (Elsevier Science, New York, 1988), Vol. XXVI, pp. 349–393. [CrossRef]
  5. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991). [CrossRef] [PubMed]
  6. J. G. Fujimoto, M. E. Brezinski, G. J. Tearney, S. A. Boppart, B. E. Bouma, M. R. Hee, J. F. Southern, E. A. Swanson, “Optical biopsy and imaging using optical coherence tomography,” Nature Med. 1, 970–972 (1995). [CrossRef] [PubMed]
  7. A. F. Fercher, “Optical coherence tomography,” J. Biomed. Opt. 1, 157–173 (1996). [CrossRef] [PubMed]
  8. G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Bopart, C. Pitris, J. F. Southern, J. G. Fujimoto, “In vivo endoscopic optical biopsy with optical coherence tomography,” Science 276, 2037–2039 (1997). [CrossRef] [PubMed]
  9. J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, J. G. Fujimoto, “Optical coherence microscopy in scattering media,” Opt. Lett. 19, 590–593 (1994). [CrossRef] [PubMed]
  10. M. Kempe, W. Rudolph, E. Welsch, “Comparative study of confocal and heterodyne microscopy for imaging through scattering media,” J. Opt. Soc. Am. A 13, 46–52 (1996). [CrossRef]
  11. E. A. Swanson, D. Huang, M. R. Hee, J. G. Fujimoto, C. P. Lin, C. A. Puliafito, “High-speed optical coherence domain reflectometry,” Opt. Lett. 17, 151–153 (1992). [CrossRef] [PubMed]
  12. B. Bouma, G. J. Tearney, S. A. Boppart, M. R. Hee, M. E. Brezinski, J. G. Fujimoto, “High-resolution optical coherence tomography imaging using a mode-locked Ti:Al2O3 laser source,” Opt. Lett. 20, 1486–1488 (1995). [CrossRef] [PubMed]
  13. S. A. Boppart, B. E. Bouma, C. Pitris, J. F. Southern, M. E. Brezinski, J. G. Fujimoto, “In vivo optical coherence tomography cellular imaging,” Nature Med. 4, 861–867 (1998). [CrossRef]
  14. W. Drexler, U. Morgner, F. X. Kärtner, C. Pitris, S. A. Boppart, X. D. Li, E. P. Ippen, J. G. Fujimoto, “In vivo ultrahigh-resolution optical coherence tomography,” Opt. Lett. 24, 1221–1223 (1999). [CrossRef]
  15. E. Beaurepaire, L. Moreaux, F. Amblard, J. Mertz, “Combined scanning optical coherence and two-photon-excited fluorescence microscopy,” Opt. Lett. 24, 969–971 (1999). [CrossRef]
  16. A. Gh. Podoleanu, G. M. Dobre, D. J. Webb, D. A. Jackson, “Coherence imaging by use of a Newton rings sampling function,” Opt. Lett. 21, 1789–1791 (1996). [CrossRef] [PubMed]
  17. G. S. Kino, S. C. Chim, “Mirau correlation microscope,”Appl. Opt. 29, 3775–3783 (1990). [CrossRef] [PubMed]
  18. M. Davidson, K. Kaufman, I. Mazor, F. Cohen, “An application of interference microscopy to integrated circuit inspection and metrology,” in Integrated Circuit Metrology, Inspection, and Process Control, K. M. Monahan, ed., Proc. SPIE775, 233–247 (1987). [CrossRef]
  19. A. Dubois, J. Selb, L. Vabre, A. C. Boccara, “Phase measurements with wide-aperture interferometers,” Appl. Opt. 39, 2323–2331 (2000). [CrossRef]
  20. J. C. Canit, J. Badoz, “New design for a photoelastic modulator,” Appl. Opt. 22, 592–594 (1983). [CrossRef] [PubMed]
  21. A. C. Boccara, F. Charbonnier, D. Fournier, P. Gleyzes, “Procédé et dispositif de détection synchrone multicanal,” French patentFR90.092255 (08255, June29, 1990) and international extensions.
  22. A. Dubois, “Phase-map measurements by interferometry with sinusoidal phase modulation and four integrating buckets,” J. Opt. Soc. Am. A 18, 1972–1979 (2001). [CrossRef]
  23. J. W. Goodman, Statistical Optics (Wiley, New York, 1984), Chap. 5.
  24. A. K. Dunn, V. P. Wallace, M. Coleno, M. W. Berns, B. J. Tromberg, “Influence of optical properties on two-photon fluorescence imaging in turbid samples,” Appl. Opt. 39, 1194–1201 (2000). [CrossRef]
  25. K. K. Bizheva, A. M. Siegel, A. K. K. Dunn, D. A. Boas, “Detection of multiply scattered light in optical coherence microscopy,” in Waves and Imaging through Complex Media, P. Sebbah, ed. (Kluwer Academic, Dordrecht, The Netherlands, 2001), pp. 277–298. [CrossRef]
  26. M. Born, E. Wolf, Principles of Optics, 6th ed. (Pergamon, Oxford, 1993).
  27. A. Dubois, A. C. Boccara, M. Lebec, “Real-time reflectivity and topography imagery of depth-resolved microscopic surfaces,” Opt. Lett. 24, 309–311 (1999). [CrossRef]

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