We describe an original microscope for high-resolution optical coherence tomography applications. Our system is based on a Linnik interference microscope with high-numerical-aperture objectives. Lock-in detection of the interference signal is achieved in parallel on a CCD by use of a photoelastic birefringence modulator and full-field stroboscopic illumination with an infrared LED. Transverse cross-section (en-face, or XY) images can be obtained in real time with better than 1-μm axial (Z) resolution and 0.5-μm transverse (XY) resolution. A sensitivity of ~80 dB is reached at a 1-image/s acquisition rate, which allows tomography in scattering media such as biological tissues.
© 2002 Optical Society of America
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.3170) Microscopy : Interference microscopy
Arnaud Dubois, Laurent Vabre, Albert-Claude Boccara, and Emmanuel Beaurepaire, "High-Resolution Full-Field Optical Coherence Tomography with a Linnik Microscope," Appl. Opt. 41, 805-812 (2002)