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Applied Optics

Applied Optics


  • Vol. 41, Iss. 7 — Mar. 1, 2002
  • pp: 1408–1417

Light scattering by aggregated red blood cells

Stephanos V. Tsinopoulos, Euripides J. Sellountos, and Demosthenes Polyzos  »View Author Affiliations

Applied Optics, Vol. 41, Issue 7, pp. 1408-1417 (2002)

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In low flow rates, red blood cells (RBCs) fasten together along their axis of symmetry and form a so-called rouleaux. The scattering of He-Ne laser light by a rouleau consisting of n (2 ≤ n ≤ 8) average-sized RBCs is investigated. The interaction problem is treated numerically by means of an advanced axisymmetric boundary element—fast Fourier transform methodology. The scattering problem of one RBC was solved first, and the results showed that the influence of the RBC’s membrane on the scattering patterns is negligible. Thus the rouleau is modeled as an axisymmetric, homogeneous, low-contrast dielectric cylinder, on the surface of which appears, owing to aggregated RBCs, a periodic roughness along the direction of symmetry. The direction of the incident laser light is considered to be perpendicular to the scatterer’s axis of symmetry. The differential scattering cross sections in both perpendicular and parallel scattering planes and for all the scattering angles are calculated and presented in detail.

© 2002 Optical Society of America

OCIS Codes
(000.4430) General : Numerical approximation and analysis
(170.1530) Medical optics and biotechnology : Cell analysis
(290.5850) Scattering : Scattering, particles

Original Manuscript: May 21, 2001
Revised Manuscript: September 28, 2001
Published: March 1, 2002

Stephanos V. Tsinopoulos, Euripides J. Sellountos, and Demosthenes Polyzos, "Light scattering by aggregated red blood cells," Appl. Opt. 41, 1408-1417 (2002)

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  1. A. L. Copley, “On erythrocyte aggregation and disaggregation,” Clin. Hemorheol. 7, 3–14 (1987).
  2. S. Chen, G. Barshtein, B. Gavish, Y. Mahler, S. Yedgar, “Monitoring of red blood cell aggregability in a flow chamber by computerized image analysis,” Clin. Hemorheol. 14, 497–508 (1994).
  3. A. Gaspar-Rosas, G. B. Thurston, “Erythrocyte aggregate rheology by transmitted and reflected light,” Biorheology 25, 471–487 (1988). [PubMed]
  4. M. Donner, M. Siadat, J. F. Stoltz, “Erythrocyte aggregation: approach by light scattering determination,” Biorheology 25, 367–375 (1998).
  5. G. B. Thurston, “Light transmission through blood in oscillatory flow,” Biorheology 27, 687–700 (1990).
  6. E. Muralidharan, M. Singh, “Influence of diabetes mellitus on the aggregation mechanism as analyzed by He-Ne laser light scattering,” Clin. Hemorheol. 11, 205–216 (1991).
  7. E. Muralidharan, “Simultaneous determination of hematocrit aggregate size and sedimentation velocity by He-Ne laser scattering,” Biorheology 31, 587–599 (1994). [PubMed]
  8. E. Muralidharan, N. Tateishi, N. Maeda, “A new laser photometric technique for the measurement of erythrocyte aggregation and sedimentation kinetics,” Biorheology 31, 277–285 (1994). [PubMed]
  9. M. R. Hardeman, P. T. Goedhart, J. G. G. Dobbe, K. P. Lettinga, “Laser-assisted optical rotational) cell analyzer (L.O.R.C.A). I. A new instrument for measurement of various structural hemorheological parameters,” Clin. Hemorheol. 14, 605–618 (1994).
  10. M. Singh, M. Kumaravel, “Influence of jaundice on aggregation process and deferability of erythrocytes,” Clin. Hemorheol. 15, 273–290 (1995).
  11. V. Twersky, “Absorption and multiple scattering by biological suspensions,” J. Opt. Soc. Am. 60, 1084–1093 (1970). [CrossRef] [PubMed]
  12. V. S. Lee, L. Tarassenko, “Absorption and multiple scattering by suspensions of aligned red blood cells,” J. Opt. Soc. Am. 8, 1135–1141 (1991). [CrossRef]
  13. G. N. Constantinides, D. Gintides, S. E. Kattis, K. Kiriaki, C. A. Paraskeva, A. C. Payatakes, D. Polyzos, S. V. Tsinopoulos, S. N. Yannopoulos, “Computation of light scattering by axisymmetric nonspherical particles and comparison with experimental results,” Appl. Opt. 37, 7310–7319 (1998). [CrossRef]
  14. Y. C. Fung, Biomechanics: Mechanical Properties of Living Tissues (Springer-Verlag, New York, 1981).
  15. G. J. Streekstra, A. G. Hoekstra, E. J. Nijohof, R. M. Heethaar, “Light scattering by red blood cells in ektacytometry: Fraunhofer versus anomalous diffraction,” Appl. Opt. 32, 2266–2272 (1993). [CrossRef] [PubMed]
  16. A. M. K. Nilsson, P. Alsholm, A. Karlsson, S. Andersson-Engels, “T-matrix computations of light scattering by red blood cells,” Appl. Opt. 37, 2735–2748 (1998). [CrossRef]
  17. P. Mazeron, S. Muller, “Light scattering by ellipsoids in a physical optics approximation,” Appl. Opt. 35, 3726–3735 (1996). [CrossRef] [PubMed]
  18. G. S. Stamatakos, D. Yova, N. K. Uzunoglu, “Integral equation model of light scattering by an oriented monodisperse system of triaxial dielectric ellipsoids: application in ektacytometry,” Appl. Opt. 36, 6503–6512 (1997). [CrossRef]
  19. A. N. Shvalov, J. T. Soini, A. V. Chernyshev, P. A. Tarasov, E. Soini, V. P. Maltsev, “Light-scattering properties of individual erythrocytes,” Appl. Opt. 38, 230–235 (1999). [CrossRef]
  20. P. Mazeron, S. Muller, “Dielectric or absorbing particles: EM surface fields and scattering,” J. Opt. 29, 68–77 (1998). [CrossRef]
  21. S. V. Tsinopoulos, D. Polyzos, “Scattering of He-Ne laser light by an average-sized red blood cell,” Appl. Opt. 38, 5499–5510 (1999). [CrossRef]
  22. S. V. Tsinopoulos, S. E. Kattis, D. Polyzos, “Three dimensional boundary element analysis of electromagnetic wave scattering by penetrable bodies,” Comput. Mech. 21, 306–315 (1998). [CrossRef]
  23. S. V. Tsinopoulos, S. E. Kattis, D. Polyzos, “An advanced BE/FFT methodology for solving electromagnetic wave scattering problems with axisymmetric dielectric particles,” Eng. Anal. Boundary Elements 23, 155–165 (1999). [CrossRef]
  24. R. D. Haracz, L. D. Cohen, A. Cohen, “Scattering of linearly polarized light randomly oriented cylinders and spheroids,” J. Appl. Phys. 58, 3322–3327 (1985). [CrossRef]
  25. M. I. Mishchenko, L. D. Travis, A. Macke, “Scattering of light by polydisperse, randomly oriented, finite circular cylinders,” Appl. Opt. 35, 4927–4940 (1996). [CrossRef] [PubMed]
  26. Y. Liu, W. P. Arnott, J. Hallett, “Anomalous diffraction theory for arbitrarily oriented finite circular cylinders and comparison with exact T-matrix results,” Appl. Opt. 37, 5019–5030 (1998). [CrossRef]
  27. J. A. Stratton, Electromagnetic Theory (McGraw-Hill, New York, 1941).
  28. R. A. Meyer, “Light scattering from red blood cell ghosts: sensitivity of angular dependent structure to membrane thickness and refractive index,” Appl. Opt. 16, 2036–2038 (1977). [CrossRef] [PubMed]

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